Targeting Proteins

Nls players cargo proteins with nls import proteins

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NLS
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PLAYERS Cargo Proteins With NLS Import Proteins Ran Ran-GEF Nuclear Import
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Ran: GTPase in Nuclear Transport G protein switches Two confirmations GTP-bound GDP-bound GTPase hydrolyzes GTP to GDP; slow enzyme Modifying Proteins Guanine nucleotide exchange factors (GEF) GDP GTP Nuclear GTPase Activating Protein (GAP) ***this protein is not a GTPase!! GTP GDP Cytoplasmic
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Nuclear Import Importin binds to cargo Increased affinity for Ran-GDP Increased affinity for FG nups moving from cytoplasm to nucleus Ran-GDP contacts Ran-GEF and changes to Ran-GTP Decreased affinity for cargo complex
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Nuclear Export PLAYERS Cargo Proteins With NES Export Proteins Ran Ran-GAP
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Nuclear Export Exportin binds to cargo Increased affinity for Ran-GTP Increased affinity for FG nups moving from nucleus to cytoplasm Ran-GTP contacts Ran-GAP which accelerates GTP hydrolysis Ran-GTP converted to Ran-GDP Decreased affinity for cargo complex
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Facilitated Diffusion: Brownian Gate Model 1. Selective Accumulation 2. Directional Diffusion 3. Trapping 4. Vectorial Release Brownian Motion Gatekeeper
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Mitochondria Power house of cell Outer membrane simple bilayer permeable to ions and small molecules Inner membrane IMPERMEABLE to all material except through carrier channels
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NLS PLAYERS Cargo Proteins With NLS Import Proteins Ran Ran...

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