bio lab 2 bacterial identification lab

Another lane should be that of the negative control

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control should be known. Another lane should be that of the negative control which should show no result. The last lane will be that of the sample. (the drawing is shown at the end) 12) The gel electrophoresis would purify the desired PCR product because the smaller fragments of DNA will move farther away from the well on the gel. This is because the shorter fragments can move better through the pores of the gel. One can then cut out the particular strand of DNA they want from the gel once it is separated. 13) The sequencing brew contains buffers, primers, DNA polymerases, nucleotides, the PCR product, and fluorescence-tagged terminators in suitable proportions. The PCR master mix contain water, a buffer, large quantities of the four nucleotides, large quantities of oligonucleotide DNA primers, and a heat-stable DNA polymerase, while the PCR master mix contains large quantities of both the nucleotides and the DNA primer . Differences in the two mixes are that only the sequencing brew contains fluorescence tagged terminators. This is because the aim of this step is to produce DNA fragments of varied length not identical copies. ?14) G, G C, GC T, GCT T, GCTT G, GCTTG G, GCTTGG C, GCTTGGC T, GCTTGGCT A The sequence of DNA that would be present would be GCTTGGCTA. The smaller DNA fragment would be the farthest away and the longest would be the closest to the well in electrophoresis. 15) The bacteria is Bartonella henselae. The patient could have contracted it by a vector or by an animal such as a cat, sandflies, or body lice. 16) It can be concluded that the Pax6 gene is closer in relation to the shark gene. However the worm gene is also close to the shark and mouse gene. Therefore it might be possible that the shark gene diverged from the worm gene in evolutionary history and then the mouse gene diverged from the shark gene more recently in evolution history.
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