BIOS242_Week2LabReport_MIKAELLA_VILLAR.docx

How important is sterile technique in a wetlab why

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How important is sterile technique in a wetlab? Why? It’s very important because you don’t want anything contaminating your experiment. Conclusion
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3 The lab was to conduct aseptic tests. The first one was a contamination test and the second one was an aseptic transfer tube-to-tube test. The tests required incubation in 37 degrees. The second experiment required flaming the loop to make it sterile. BACTERIAL ANATOMY Introduction The first experiment was led by the death of birds in Long Island. Health officials panicked due to the threat of the West Nile virus. Tests were done on birds and yielded numerous bacterial species, including the organism for the first experiment. The second experiment is an organism cultured from the drainage from a 17-year old’s pleural space that was filled with fluid. Procedure Experiement 1 1. Start a New Unkown and label it “Anatomy1” 2. Select “Bacterial Anatomy 1” from the Subgroup menu. 3. Click Auto-inoculation allowed. 4. Record the case study. 5. Observe the morphology of the bacteria and record the results Experiment 2 1. Start a New Unkown and label it “Anatomy2” 2. Select “Bacterial Anatomy 2” from the Subgroup menu. 3. Click Auto-inoculation allowed. 4. Record the case study. 5. Observe the morphology of the bacteria and record the results Observations and Results Gram stain result: Experiment 1- Gram-positive cocci Experiment 2- Gram-negative rods Discussion In the Discussion section, answer the following questions. Describe the morphology of each bacterium Experiment one consisted of Gram-positive cocci (they were round) Experiment 2 consisted of Gram-negative rods
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4 Describe the arrangement of the bacteria. Were the bacteria organized as
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