and second quadrant were not fully covered with colonies.
The significant of this experiment is getting to know and work with different
mediums like synthetic, complex, and differential medium, and understanding that
growth medias can be in different forms based on the growth needs. Understanding the
importance of pure culture techniques to carry out the characterize bacterial species.
And again, emphasizing how aseptic technique is important to all procedures involving
preparation of any bacterial growth. Some of the errors, burning of the loop coil
destroyed all the samples by killing the bacteria before transporting to the medium,
which led to no growth in all mediums. It would have been better to be careful with
aseptic technique and wait for the coil to cool down completely before taking sample of
the bacteria. Labeling the tubes before anything to not get everything mixed together

Mohammed Ali 2
because we had similar results but did not know which one belongs to which media.
The isolation of bacteria into single colonies it did not reach that because the last streak
was not fully in third quadrant. Preparing culture media and calculating the ingredients
are important to pharmacy filed as it uses a range of culture medias to test different
bacteria and how it how reacts with the environment. An accurate calculation of the
ingredients will help avoiding any unnecessary mistakes that might cause labs and
drugs companies a lot of money.

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- Spring '08
- Hong-Chuan Lin
- Bacteria, Mohammed Ali