Acetyl-CoA carboxylase 2-:- mutant mice are protected against fatty liver under high-fat, high-carb

Mutant mice was about 25 lower than that of the wild

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mutant mice was about 25% lower than that of the wild-type controls (Fig. 1 D ; p 0.005). Although both groups lost about 14% of their body weight after the star- vation period (Fig. 1 D ), this loss was not statistically signifi- cantly different ( p 0.15). By the end of the 48-h refeeding, the Acc2 / mutants consumed about 22% more food than the wild-type controls (7.9 versus 6.2 g/mouse, respectively; data not shown). After the 48-h refeeding, both groups of mice regained about 90% of their initial body weight before starva- tion (28.88 4.4 versus 32.16 4.5 g/mouse and 21.4 1.2 g versus 22.5 1.9 g/mouse, respectively; Fig. 1 D ). The weights of the livers were similar for both groups of mice after the 48-h refeeding (1.02 0.15 versus 0.96 0.12 g; Fig. 1 E ), but, the epididymal fat pads of the Acc2 / mutants weighed about 65% less (1.19 0.31 versus 0.45 0.09 g; p 0.04; Fig. 1, E and F ). These results suggest that the wild-type mice regained most of their white adipose tissue after being refed the FFHC diet, whereas the Acc2 / mutants mice only partially regained this tissue. When both groups of mice were fed the HFHC diet, the Acc2 / mutant mice weighed about 20% less before eating the diet and 42% less after eating the diet (Fig. 1 G ). On average, the Acc2 / mutants gained about 7.5 g/mouse (range: 20.88 0.98 to 28.3 1.3 g/mouse), whereas the wild-type controls gained about 22 g/mouse (range: 26.34 2.05 to 48.42 5.3 g/mouse; Fig. 1 G ). Under these feeding conditions, the livers of Acc2 / mutants weighed about half the weight of the livers of the wild- type controls (0.88 0.09 versus 1.75 0.5 g; p 0.018; Fig. 1 H ). However, when the ratio of liver weight to body weight was calculated for both groups, the ratio was similar ( p 0.14; Fig. 1 I ). On the other hand, the size of the epididymal fat pads of the Acc2 / mutant mice was about 38% of that of the wild-type controls ( p 0.0005) and about 50% less when the ratio of adipose tissue weight to body weight was calculated ( p 0.001; Fig. 1 I ). Under prolonged fasting (48 h), the wild-type controls lost an average of 2.9 1.01 g of their body weight, and the Acc2 / mutants lost an average of 1.6 0.40 g (Fig. 1 J ). The livers of the Acc2 / mutants weighed about 44% less ( p 0.003) and their epididymal fat pads weighed about 50% less than those of the TABLE 1 Serum metabolite data from wild-type and Acc2 / mutant mice under different dietary conditions Blood was withdrawn from tails after a 5–6-h fasting period, and serum was separated and kept at 20 °C for further analysis of the blood constituents. ND a FFHC HFHC Fast Blood constituents WT Mut WT Mut WT Mut WT Mut Glu (mg/dl) 205 23 182 9 151 10 151 9 233 19 186 13 b 74 13 71 11 Cholesterol (mg/dl) 145 22 154 22 92 15 123 9 b 204 38 270 26 177 11 149 10 b HDL cholesterol (mg/dl) 131 33 140 22 83 10 104 6 b 186 31 242 35 b 165 11 145 10 LDL cholesterol (mg/dl) 14 2 18 3 12 11 21 4 b 17 4 29 7 b 12 2 11 3 TG (mg/dl) 157 52 101 15 b 91 20 61 4 b 137 41 93 15 b 160 40 80 6 b VLDL cholesterol (mg/dl) 32 11 20 9 b 18 4 12 1 b 28 8 18 3 b 38 12 16 1 b NEFA (mEq/dl) 3.1 0.3 2.3 0.2 b 1.1 0.1 0.8 0.1 2.1 0.4 1.5 0.2 b 1.7 0.1 1.2 0.1 b a ND, normal diet; FFHC, fasted-refed; Fast, fasted for 48 h; HFHC, high-fat, high-carbohydrate diet; mut, mutant; NEFA: nonesterified fatty acids; VLDL, very low-density lipoprotein.
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  • Winter '19
  • Robert S Kiss
  • The American, Fatty acid metabolism

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