3. Create a driving question: choose one of the factors you’ve identified that can be 6
controlled in the lab and develop a testable question for your experiment. How does the rate of oxygen consumption affect the rate of carbon dioxide production, in turn affect the rate of cellular respiration? 4. What is the justification for your question, that is, why is it biologically significant, relevant, or interesting? The study of the relationship between oxygen consumption and the production of carbon dioxide should help to understand the utilization of oxygen in the atmosphere that helps the process of cellular respiration, an important function of life. By utilizing the data from the experiment, the effect that oxygen has on the production of CO 2 can be learned as well as the importance of oxygen abundance in the atmosphere that is needed for living organisms to thrive. 5. What will be the independent variable of the experiment? Describe how this variable will be manipulated in your experiment. The independent variable of the experiment for this experiment should be time in minutes, and would be manipulated to have a sample rate of one sample every 10 seconds. 6. What is the dependent variable of the experiment? Describe how the data will be collected and processed in the experiment. The dependent variable should be the amount of O 2 released vs. CO 2, measured in parts per million (ppm) per minute. The data would be collected by the transfer of the sensors to a computer software that would provide information on both the rate of consumption of O 2 and production of CO 2 . 7. Write a testable hypothesis (If...then...). If an organism is placed in an isolated chamber, then those that consume more oxygen would produce more CO 2 gas that will increase the rate of cellular respiration. 7
8. What conditions will need to be held constant in the experiment? Quantify these values where possible. One condition that must be held constant in the experiment is the environmental factor (room temperature, amount of light, etc.). Though this factor was not quantified, over the course of the run, the chamber was held at the same temperature and amount of light, allowing the trials to have considerable consistency throughout. Another condition that was held constant was the amount of objects in each trial. Having at least four organisms of each insect species should also be held constant, as it is more quantifiable. 9. How many trials will be run for each experimental group? Justify your choice. Three trials will be run for each experimental group. Having more than one trial should provide accuracy for the data collected. The rate of consumption of O 2 and CO 2 would be measured more accurately this way. 10. What will you compare or calculate? What analysis will you perform to evaluate your results and hypothesis? The rate of consumption of O 2 and production of CO 2 will be compared and calculations would be made by the process of elementary division of the ppm/min (parts per million/min) of these rates by the mass of the different insect species. The analysis made will be based solely on the comparisons between the rate of consumption of O 2 and CO 2 .
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- Summer '14