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12. Make an additional 200 mL of 15% sucrose solution in Beaker 1 by mixing the 30% stock solution with water. Use the following dilution equation to solve for the necessary amount of stock solution.M1V1= M2V2Where:V1 = Needed volume of stock solutionM1 = Sucrose concentration of stock solution = 30%V2 = Desired volume of Beaker 1 solution = 200mLAfter solving for V1, figure out the how much water is needed to bring the total volume in Beaker 1 to 200mL. Record your calculated volume below.mL of water needed = _100 mL____________mL of 30% stock sucrose solution needed = _____________Part II: Experimental ProcedureIMPORTANT:Your two “unknown” liquids should be at room temperature when you pour them into the dialysis bag.1.Prepare the following five experimental treatments (solutions in dialysis bags).BlueYour Choice #1GreenYour Choice #2WhiteFor each treatment, use the graduated cylinder to measure out 5mL of the corresponding solution (from those that you prepared earlier and your two choices of fluid). Pour those 5mLinto the prepared dialysis bag with the matching band color.Secure the tops of each bag in the same way that you secured the bottoms of each bag, using the same colored rubber bands for each bag. If you do not have enough tubing, you can skip tying a knot at the top of the bag. Make sure the top is as secure as possible using
BI205: Introductory Biology IIWeek 4 LabPage 5 of 82.Describe your “unknown” sugar solutions here.For example, you could say, “I dissolved1 tablespoon (~15 mL) of honey in 100 mL tap water, and poured 5 mL of this into one dialysis bag, and I poured 5 mL of room temperature orange juice into the other dialysis bag.” Description: I poured 5 mL of sugar free soda in one bag and regular soda in another. 3.Take a photograph of your experimental set-up (the dialysis bags in the Beaker 2), and insert it here.Photo:4.Measure the weights of your five filled dialysis bags using the digital scale. Record your measurements in the following tableTreatmentBand ColorWeight3% sucroseYellow0.1715% sucroseRed0.1930% sucroseBlue0.24Your Choice #1Green0.16Your Choice #2White0.215.Transfer your five filled dialysis bags to Beaker 1, containing your preparation of 200 mL of 15% sucrose solution. You may pour out some of this solution into a waste beaker if Beaker 1 begins to overflow when you add the dialysis bags.