Lucci_Prelab8_Monday2pm

9 parts 45ml petroleum ether 1 part 05ml acetone

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9 parts (4.5mL) Petroleum ether 1 part (0.5mL) Acetone Exercise 8.5: 19. Briefly describe what a spectrophotometer is and how it works. An instrument that measures the proportions of light of different wavelengths absorbed and transmitted by a pigment solution. It does this by passing a light of a particular wavelength through the solution. 20. Where are the sample pigments for this exercise obtained from? Spinach leaves Exercise 8.6: 21. What is the objective of this exercise? Test the necessity of CO 2 in photosynthesis using Elodea and an indicator for the presence of CO 2 . 22. Name the pH indicator used in this exercise. What color changes does this indicator exhibit? Bromothymol blue, turns yellow in the presence of carbonic acid (H 2 CO 3 ) Exercise 8.7: 23. How will photosynthetic rate be measured in this experiment? Vacuuming out the air in the intercellular spaces and then placing in a solvent and allow photosynthesis to replace the O 2 in the spaces. 24. What is the source of carbon dioxide for the experiment? 25. Identify: The dependent variable: The independent variable: 3
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Exercise 8.8: 26. Identify the following: i) the plant to be used and research why. ii) the water-soluble pigment responsible for the blue, violet, purples, and dark colors in a plant. iii) the purpose of the alcohol bath: iv) the reason for using IKI. 4
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