6 emb agar lhyehek 1 which bacteria are inhibited by

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6) EMB agar
1) Which bacteria are inhibited by EMB? Most gram positive organisms. 2) Which bacteria grows on EMB? Escherichia coli.
3) What color is produced by E.coli? Blue-black centered colonies with green metallic sheen. 4) Which color is produced by Enterococcus cloacae? Purple, mucoid colonies.
7) catalase test
1) What is produced by catalase enzyme? Detoxifies hydrogen peroxide into water and molecular oxygen. 2) What indicates the positive catalase test? If bubbles are observed after adding hydrogen peroxide.
3) Which medium do you use for this test? Nutrient agar
8) carbohydrate (sugar)fermentation tests
2) Which color(pH) indicator is used in this test? Phenol red. 3) What is the color of positive test? Yellow.
9) Gelatin test 1) Which medium do you use for this test? Nutrient gelatin medium- no agar. 2) What is positive test? If you have a positive gelatin result your tube will not resolidify, it will remain liquid. 3) Why do we need to place tubes in the ice to check positive test? To check the reaction and confirm liquefaction. Aseptic Lab Technique- Virtual Lab A) Read the Aseptic technique lab exercise from your lab manual. B) Read and view the procedure and watch the animation here . Complete self-evaluation and assignment questions. Then answer the following questions and submit it to your instructor: 1. What is the importance of using aseptic technique in a Microbiology lab?
2. What would happen if you were to insert a red-hot loop for picking the bacterial culture?
3. What is the purpose of flaming the mouth of the tube by placing it at the opening of the microincinerator?
4. Why do you sterilize your loop before picking up the bacterial culture? Why do you sterilize it again after inoculation?
5. How would you determine if your aseptic transfer was successful?
The organism produce a red/pink pigment when it is incubated.

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