Bacteriorhodopsin exists in 2 states tense t binds

Info icon This preview shows pages 40–48. Sign up to view the full content.

View Full Document Right Arrow Icon
Bacteriorhodopsin exists in 2 states : Tense (T) – binds trans -retinal Relaxed (R) – binds cis- retinal T state: Schiff Base is protonated Excitation by photon Isomerization H + transferred from Schiff base to Asp 85 R state: H + transferred from Asp 96 to Schiff base and H + from Asp 85 is transferred to Extracellular Space H + transferred from Cytoplasm to Asp 96
Image of page 40

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Photosiomerization of retinal
Image of page 41
Bacteriorhodopsin photocycle
Image of page 42

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Bacteriorhodopsin photocycle
Image of page 43
Bacteriorhodopsin photocycle
Image of page 44

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Porin Homotrimeric protein. – Each monomer is made of a 16 stranded Up/Down β Barrel. – Subunits interact by polar loop and hydrophobic side chain interactions. Periplasmic End: short and smooth Cytoplasmic End: funnel shaped, made of long loops with hydrophilic residues. Eyelet = loop connecting β 5 and β 6 extending into the central open cavity of the barrel. – Cavity is lined with charged residues, arranged in an electrochemical gradient. Fig12.7
Image of page 45
Porin Trimer
Image of page 46

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Porin – Aromatic Belt Aromatic Belt – mainly Phe and Tyr Has polar and hydrophobic character to interactive with the hydrophilic space of the membrane extracellular solution and hydrophobic internal space of the membrane.
Image of page 47
Image of page 48
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern