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17Bacterial plasmid DNA and bacteriophage DNA are commonly used ______________ to introduce foreign DNA into a bacterium.Section: 5.218The enzyme _________________ can be used to add nucleotides to the 3' end of DNA.Section: 5.219A gene’s function can be studied by inactivating the gene by a process known as gene disruptionor _______________.Section: 5.320cDNA, attached to a microscope slide forms a _________________ used to study gene expression levels.Section: 5.4Multiple-Choice Questions21The biological role of restriction enzymes in bacteria is to A)repair DNA. D)All of the above. B)induce DNA crossover. E)None of the above. C)cleave foreign DNA. Ans: C Section: 5.122Which of the following DNA sequences contains a 48 base palindromic site? (Note: Only one strand is shown.) Section: 5.123What do Southern, Northern, and Western blots detect, respectively? Section: 5.124The specificity or strigency of a PCR reaction can be controlled by altering the reaction Section: 5.125Each cycle in PCR includes A)DNA synthesis.B)hybridization of primers.C)strand separation.D)All of the above. E)None of the above. 3
Chapter 5 Exploring Genes and GenomesAns: D Section: 5.126The first three bases of the 6-base recognition cleavage site of HindIII are AAG. What is the complete sequence of this 6 bp site?Section: 5.127Plasmids used in recombinant DNA technology typically Section: 5.228A polylinker site contains Section: 5.229Why are met and trp often used to design DNA probes from amino acid sequences? A)They are not degenerate and have single codons. B)Met is the first amino acid in the protein chain. C)Both are used often in proteins. D)All of the above. E)None of the above. Ans: A Section: 5.2