Bacterial plasmid DNA and bacteriophage DNA are commonly used to introduce

Bacterial plasmid dna and bacteriophage dna are

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17Bacterial plasmid DNA and bacteriophage DNA are commonly used ______________ to introduce foreign DNA into a bacterium. Section: 5.2 18The enzyme _________________ can be used to add nucleotides to the 3' end of DNA. Section: 5.2 19A gene’s function can be studied by inactivating the gene by a process known as gene disruptionor _______________. Section: 5.3 20cDNA, attached to a microscope slide forms a _________________ used to study gene expression levels. Section: 5.4 Multiple-Choice Questions 21The biological role of restriction enzymes in bacteria is to A)repair DNA. D)All of the above. B)induce DNA crossover. E)None of the above. C)cleave foreign DNA. Ans: C Section: 5.1 22Which of the following DNA sequences contains a 48 base palindromic site? (Note: Only one strand is shown.) Section: 5.1 23What do Southern, Northern, and Western blots detect, respectively? Section: 5.1 24The specificity or strigency of a PCR reaction can be controlled by altering the reaction Section: 5.1 25Each cycle in PCR includes A)DNA synthesis.B)hybridization of primers.C)strand separation.D)All of the above. E)None of the above. 3
Chapter 5 Exploring Genes and Genomes Ans: D Section: 5.1 26The first three bases of the 6-base recognition cleavage site of HindIII are AAG. What is the complete sequence of this 6 bp site? Section: 5.1 27Plasmids used in recombinant DNA technology typically Section: 5.2 28A polylinker site contains Section: 5.2 29Why are met and trp often used to design DNA probes from amino acid sequences? A)They are not degenerate and have single codons. B)Met is the first amino acid in the protein chain. C)Both are used often in proteins. D)All of the above. E)None of the above. Ans: A Section: 5.2

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