2 use the lamp rheostat control to set the light to a

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2. Use the Lamp Rheostat Control to set the Light to a comfortable Level. 3. Check that the 10X Objective -- not the 3.2X Objective -- is clicked into place and double-check that the Condenser Auxiliary Lens is in Position. 4. Mark a "P" on a Slide with a Sharpie and place it on the Stage. 5. Fully raise the Condenser with the Condenser Focusing Knob. 6. Focus on the “P” with the Fine and Coarse Focus Knobs. 7. Once in focus, use the Mechanical Stage Controls to move the “P” out of the way (by moving it to the Left or Right, but keep the Slide in the Optical Pathway). 8. Use the Field Diaphragm Control near the “Zeiss” Logo on the Base of the Microscope to partially close down the Field Diaphragm while looking through the Microscope. You’ll see a Polygon. 9. Focus this Polygon using the Condenser Focusing Knob. 10. Open-up the focused Polygon so it’s opened-up as much as possible but still Visible at the Edges of the Field of View. 11. Center the Polygon with the Silver Condenser Centering Screws on the Condenser. 12. Adjust the Field Diaphragm until the Polygon just fully fills the Field of View. This should be ~14 on the Field Diaphragm Scale. 13. Slowly remove the Right Eyepiece by carefully pulling it straight out. Set the Eyepiece down Right-Side-Up and away from the Edge of the Bench. 14. Adjust the Condenser Diaphragm Control on the Condenser Diaphragm such that 3/4 of the Field of View is filled with Light (i.e. close-down the Condenser Diaphragm by about 1/4). 15. Replace the Right Eyepiece. 16. Use the Mechanical Stage Controls to move the “P” back into Place. If the P is still in focus, you did it! If the P is not in Focus, you screwed-up!
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  • Winter '09
  • MANN
  • Eyepiece, field diaphragm, Condenser Diaphragm Control, Condenser Auxiliary Lens, Zeiss Microscope Parts

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