10mL undiluted 100 units/mL enzyme solution from exercise 6.1iii) substrate concentration to be used.40 mL 1% H2O2solution = 0.294M H2O2iv) independent variable. pH levelv) dependent variable. Rate of reaction18.Describe how each pH variable is prepared. 10 mL undiluted enzyme solution30 mL buffer solution of the appropriate pH40 mL 1% H2O2solutionExercise 6.6:19.What is the objective of this exercise?To determine the effect of introducing an enzyme inhibitor on enzyme activity.20.Identify the following:i) inhibitor to be tested. Hydroxylamineii) Enzyme concentration to be used.5 mL undiluted 100 units/mL enzyme solutioniii) substrate concentration to be used.40 mL 1% H2O2solutioniv) independent variable. Presence of hydroxylaminev) dependent variable. Rate of reation21.How exactly does this inhibitor work?Hydroxylamine attaches to the iron atom (a part of the catalase molecule) and thereby interferes with the formation of the enzyme-substrate complex.Tip for the Challenge Qs:Before answering Qs 1, 2, 4, and 5, read the following articles. Either use “control and click” to follow the link or copy and paste it into your web browser.http://www.sciam.com/article.cfm?id=experts-why-cut-apples-turn-brownhttp://recipes.howstuffworks.com/question168.htm
This is the end of the preview.
access the rest of the document.
As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.
Temple University Fox School of Business ‘17, Course Hero Intern
I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.
University of Pennsylvania ‘17, Course Hero Intern
The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.