10ml undiluted 100 unitsml enzyme solution from

Info icon This preview shows page 3. Sign up to view the full content.

View Full Document Right Arrow Icon
10mL undiluted 100 units/mL enzyme solution from exercise 6.1 iii) substrate concentration to be used. 40 mL 1% H 2 O 2 solution = 0.294M H 2 O 2 iv) independent variable. pH level v) dependent variable. Rate of reaction 18. Describe how each pH variable is prepared. 10 mL undiluted enzyme solution 30 mL buffer solution of the appropriate pH 40 mL 1% H 2 O 2 solution Exercise 6.6: 19. What is the objective of this exercise? To determine the effect of introducing an enzyme inhibitor on enzyme activity. 20. Identify the following: i) inhibitor to be tested. Hydroxylamine ii) Enzyme concentration to be used. 5 mL undiluted 100 units/mL enzyme solution iii) substrate concentration to be used. 40 mL 1% H 2 O 2 solution iv) independent variable. Presence of hydroxylamine v) dependent variable. Rate of reation 21. How exactly does this inhibitor work? Hydroxylamine attaches to the iron atom (a part of the catalase molecule) and thereby interferes with the formation of the enzyme-substrate complex. Tip for the Challenge Qs: Before answering Qs 1, 2, 4, and 5, read the following articles. Either use “control and click” to follow the link or copy and paste it into your web browser. http://www.sciam.com/article.cfm?id=experts-why-cut-apples-turn-brown http://recipes.howstuffworks.com/question168.htm
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern