This phenomenon was called transformation Does this experiment demonstrate that

This phenomenon was called transformation does this

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virulent strain genetically transformed the non-virulent strain into virulent bacteria. This phenomenon was called transformation. Does this experiment demonstrate that DNA is the genetic material? NO b) What experiment demonstrated that DNA was the transforming material. Give a short explanation (only two sentences are required). (See fig 10.3) The experiments by Avery, MacLeod, and McCarty demonstrated that DNA was the genetic material. By using RNase (which destroys RNA) and protease (which destroys proteins) and DNase (which destroys DNA) they found that only the tube containing DNase did not produce transformation, thus revealing that DNA was the transforming principle. 6. What facts must be true in double stranded DNA? (Circle all that are correct) a) the amount of purines (A+G) is equal to the amount of pyrimidines (C+T) b) the amount of A = T c) the amount of G = C d) (A + T) / (G + C) = 1 7. The (A + T) / (G + C) ratio in the DNA of E. coli is 1.00. Is this information sufficient to decide whether the DNA is likely to be double or single stranded? If not, state the kind of information that is required to determine the strandedness of DNA. The information provided is insufficient to determine whether the DNA is ss or ds. The other information necessary is the ratio of purines to pyrimidines or (A=T and G =C) 8. T2 bacteriophage DNA is about 52µm long. The distance between base pairs is 3.4 A. The average molecular weight of a nucleotide pair is 660. If the molecule is a bouble helix throughout its length, what is its molecular weight? Length of the double helix = 52 µm = 52 x 10 -6 m = 5.2 x 10 -5 m Distance between two pairs = 3.4 A = 3.4 x 10 -10 m Number of nucleotide pairs = 5.2 x 10 -5 / 3.4 x 10 -10 = 152941 Therefore the molecular with of the molecule = 152941 x 660 = 100941060 =1.009 x 10 9
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  • Spring '11
  • dr
  • DNA, Pyrimidines

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