Benazzoug Y, Logeart D, Labat-Robert J, et al: Derivatized dextrans modulate collagen synthesis in aortic smooth muscle cells. Biochem Pharmacol 49:847-853, 1995 6. Benoit J, Meddahi A, Ayoub N, et al: New healing agent for colonic anastomosis. Int J Colorectal Dis 13:78- 81, 1998 7. Dam-Hieu P, Lacroix C, Said G, et al: Reduction of postoperative perineural adhesions by Hyaloglide gel: an experimental study in the rat sciatic nerve. Neurosurgery 56:425-433; discussion 425-433, 2005 8. Davison SP, McCaffrey TV, Porter MN, et al: Improved nerve regeneration with neutralization of trans- forming growth factor-beta1. Laryngoscope 109:631-635, 1999 9. Dumanian GA, McClinton MA, Brushart TM: The effects of free fat grafts on the stiffness of the rat sciatic nerve and perineural scar. J Hand Surg [Am] 24:30-36, 1999 10. Finsterbush A, Porat S, Rousso M, et al: Prevention of peripheral nerve entrapment following extensive soft 106
8 General Discussion, Conclusions and Research Perspectives 109 32. Ruch DS, Spinner RM, Koman LA, et al: The histological effect of barrier vein wrapping of peripheral nerves. J Reconstr Microsurg 12:291-295, 1996 33. Smit X, Stefan de Kool B, Walbeehm ET, et al: Magnetoneurography: recording biomagnetic fields for quantitative evaluation of isolated rat sciatic nerves. J Neurosci Methods 125:59-63, 2003 34. Smit X, van Neck JW, Afoke A, et al: Reduction of neural adhesions by biodegradable autocrosslinked hyaluronic acid gel after injury of peripheral nerves: an experimental study. J Neurosurg 101:648-652, 2004 35. Smit X, van Neck JW, Ebeli MJ, et al: Static footprint analysis: a time-saving functional evaluation of nerve repair in rats. Scand J Plast Reconstr Surg Hand Surg 38:321-325, 2004 36. Tardieu M, Gamby C, Avramoglou T, et al: Derivatized dextrans mimic heparin as stabilizers, potentiators, and protectors of acidic or basic FGF. J Cell Physiol 150:194-203, 1992 37. Walbeehm ET, Dudok van Heel EB, Kuypers PD, et al: Nerve compound action current (NCAC) measure- ments and morphometric analysis in the proximal segment after nerve transection and repair in a rabbit model. J Peripher Nerv Syst 8:108-115, 2003 38. Zimowska M, Constantin B, Papy-Garcia D, et al: Novel glycosaminoglycan mimetic (RGTA, RGD120) contributes to enhance skeletal muscle satellite cell fusion by increasing intracellular Ca2+ and calpain activity. J Cell Physiol 205:237-245, 2005 108
In conclusion, footprint analysis should only serve as additional evaluation method of nerve injury, providing indirect evidence of the recovery process of the nerve at the site of injury. Static footprint analysis is comparable to the traditional walking track analysis but easier and more time efficient. The aim in chapter 3 was to develop and validate a set up enabling accurate quan- tification of the conduction capacity of a rat sciatic nerve. In vivo neurophysiological measurements are heavily prone to errors, i.e. recording distances and temperature, especially in small sized animals. In this chapter, a setup is described, developed to accu- rately make ex vivo MNG measurements on rat sciatic nerves. Biphasic stimulation with optimized grounding reduced the stimulus artifact. The mean variability (<5%) of
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