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(f.)Based upon the steps you performed to determine the size of the Parameciumspecimen using the ocular reticle and the stage micrometer, list a set of brief instructions to determine the actual size (in SI units) of any object. Report the calibration of reticle units you determined for each objective in the table below as well. 25. 10. 2.3 N/O
Group Members: Erin Armstrong. Nathaniel Belachew. Patrick Dunne. Diego Garcia Exercise: 2 Date:14 February 2013 LI Sanjeev Namjoshi Unique 49150 Day: Tuesday Room: PAI 1.08 Lab Start Time: 6:00 PM Exercise 1 Page 4of 7N/O indicates not observed. The lab did not include oil immersion microscopy. (3)INDIVIDUAL:Paramecia(permanent dry and fresh wet mounts) (a.)Based on your experience in this exercise, report in tabular form at least two advantages and two disadvantages of permanent dry mounts compared with wet mounts for determining features of specimens as observed by brightfield microscopy. Advantages / Disadvantages Wet mounts Dry mounts Advantage- Wet mount’s movement Wet mount slides allow the observer to see the specimen’s organelles alive and in use. There is no internal movement of structures within the cell, and the cellular movement cannot be observed. Advantage- Dry mount’s lack of movement The specimen may move out of the field of view, so higher magnifications are difficult to obtain. Specimen does not move. Can be observed at higher magnifications without having to move the stage. Advantage Wet mount’s cell is alive A properly prepared wet mount Parameciaslide will contain several live Paramecia, so they can be observed as if they were in the natural habitat of water. Since dry mount slides are permanent and use dyes, the Paramecia are dead when observed. Advantage Dry mount’s cell has better contrast Since dyes often kill the specimen, wet mount slides often do not have their specimens dyed, so the contrast between cellular structures is not very visible. Permanent dry mount slides include dyes that stain cytoplasmic structures, so they can be more easily seen.
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Biology, Null hypothesis, Statistical hypothesis testing, Statistical significance, LI Sanjeev Namjoshi, Sanjeev Namjoshi Unique, Diego Garcia Exercise