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Dna polymerase enzyme that facilitates addition of

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DNA polymerase : enzyme that facilitates addition of nucleotides in building the new DNA strand. Can only adds nucleotides at the free 3’ end. Q: Why is this important? Image: DNA Detail Diagram: Madprime From the Virtual Cell Biology Classroom on ScienceProfOnline.com
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DNA Replication: Leading & Lagging Strand ________ Strand Synthesis proceeds smoothly as the replication fork unzips. ________ Strand Synthesis away from the replication fork (Okazaki fragments); joined by DNA ligase. Replication Fork Diagram : Mariana Ruiz From the Virtual Cell Biology Classroom on ScienceProfOnline.com
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Let’s Practice How Leading & Lagging Daughter Strands Are Built Within the Replication Bubble 5’ Replication Fork Replication Fork Now lets look at how replication of the leading and lagging strands occurs at each of the two replication forks within the replication bubble: 1. Label each end of the parent strands as either 5’ or 3’. 2. Start a RNA primer for each daughter strand and label its 5’ and 3’ ends. 3. Show how new strands are built (continuously or discontinuously). ?’ ?’ ?’ RNA Primers ?’ ?’ ?’ ?’ From the Virtual Cell Biology Classroom on ScienceProfOnline.com
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Reminder…Why is the DNA copied? Replication occurs prior to cell division , because the new, daughter cell will also need a complete copy of cellular DNA . Image: Types of Cell Division , Saperaud Wiki From the Virtual Cell Biology Classroom on ScienceProfOnline.com
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If binary fission creates clones… …then: Why isn’t there just one type of bacteria? How do bacteria change (for example develop resistance to antibiotics)? Genetic Diversity in Prokaryotes Images: Binary Fission , JW Schmidt From the Virtual Cell Biology Classroom on ScienceProfOnline.com
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Replication Mistakes: _________ of Genes Change in the nucleotide base sequence of a genome; rare. Almost always bad news, but... Rarely leads to a protein having a novel property that improves ability of organism and its descendants to survive and reproduce. Images: Blinky & Bart, Matt Groening From the Virtual Cell Biology Classroom on ScienceProfOnline.com
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Mutation and Bacterial Change _________ __________= When a microorganism is able to survive exposure to an antibiotic. Genetic mutation in bacteria can produce resistance to antimicrobial drugs (example: beta-lactamase).
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