In this experiment beer lambert equation that changes

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screening. In this experiment, “Beer- lambert equation that changes in absorbance is equal to extinction coefficient*path length(of cuvette)* concentration in mol. /L.” Hence, the absorbance of P-nitrophenol over time will be determined in this experiment which gives us the absorbance 405nm, and values are used to determine C and then calculate the rate of reaction and the total enzyme unit(U) in the reaction for the given enzyme for a given volume over time. The enzyme activity is the mole that is converted per unit time. Materials & Methods materials 1m Tris-HCl pH 8.0 10mg/Ml Methyl Parathion Enzyme Incubator Cuvette Cuvette spectrophotometer Paraffin Square Methods: a blank was created by the instructor and the spectrophotometer was operated. Also, a master mix in cuvette was created by the instructor. 895ul 50mM Tris HCL 5uL 10mg/ml Methyl parathion 100ul Enzyme
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BTECH 3100 Oweh 2 Method The enzyme was added and mixed as close as to spectrophotometer read as possible. The top of cuvette Para filmed and gripped the top and bottom of the cuvette with thumb and fore finger and inverted gently 3-5x to mix the reagents. The cuvette top is Para filmed properly to avoid leakage. The OD was measured in spectrophotometer at 405nm absorbance. The mix was incubated at 37 degrees Celsius for 5 min and OD measured again and recorded again at 405nm The incubation is repeated for another 5 minutes increments until 30 minutes total incubation time: and values recorded as read. The empty enzyme cuvette is discarded into biohazard Time OD: 45 0 0.0202 5 0.4224 10 0.9644 15 1.1579 20 1.2464 25 1.2935 30 1.3316
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