t of Abs in there you have to measure how well that Ab can bind strength of the

T of abs in there you have to measure how well that

This preview shows page 65 - 67 out of 70 pages.

t of Ab’s in there, you have to measure how well that Ab can bind (= strength of the Ab) Young Pre-fed 22% protein diet titer Pre-fed 28% protein diet titer Dilution of the blood 40-80 fold dilution 27 33 80-160 fold dilution 5 34 Results: Showed that even with the high dilution, the strength of the Ab didn’t change; it was still very strong. The birds on a higher protein diet, then challenged with a protein stress (the ability to make Ab’s from a virus) faired much better 3) Another issue in trying to determine if higher prot diets make a difference with protein stresses is that they use supra-levels of high and low prot diets ( e.g.- 30% vs. 60% prot diets.) o Major criticism: they’re really comparing a high protein with a super high protein diet. 60% protein diets aren’t compatible with a balanced diet in humans & highly unusual Under those circumstances the super high protein diets wouldn’t make a difference
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SFLORES_4/7/2014 66 So investigators began carrying out studies feeding normal amounts of protein and slightly higher amounts. HIGH PROT DIET = LONGER ADAPTATION TIME & MISC LOSSES - Study done in young college students: feeding high protein diets that could be achieved in your own lifestyle. Measured N retention over long periods of time. o Done under intensive conditions (put in a metabolic ward for several weeks) Environment was heavily controlled: & they tried to account for virtually all N losses. (B/c normal N balance studies tend to overestimate food intake and underestimate excretion b/c they don't account for miscellaneous losses) o Fed diets with either 12g N or 36g N for 50d 50d = enough time to overcome adaptation pd of new, higher protein intake 12 g N x 6.25 = 75g protein which is easily achievable in the diet 36g N = 225g which is still achievable for those who workout a lot or do a lot of physical performance o Results: on 36 g N, even after 50d, there was a persistent + N balance/retention of ~1.6g N/d (~10g protein). You would expect after 50d that adaptation pd is done, oxidation should b/c of all the extra AAs and be picked up as urea in the urine. Instead there was a persistent + N balance by ~10g protein/d and if you convert it to protein body mass you multiply by 4-5x to account for water Theoretically if putting on more lean body mass with 10g extra protein/d then they have to put on ~40-50g BW/d and you should be able to pick this up in BW changes but they didn't. They found that on 36g N (225 g protein) the losses from skin, hair and nails were 2x as high than on normal protein intake. o So on high protein intake hair growth , you slough more skin and N loss that way. Also blood urea N levels (a proxy for determining quality of a protein). Why? With extra N coming into the body w/ excess protein, you re going to make more urea But no change in Creatinine or creatine - With no change in BW, the authors speculated that extra retention was an accommodation and that there’s an in the retention of this N at the expense of cellular water o In other wo rds if you’re retaining extra N, accounting for
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  • Spring '14
  • Knutson,MitchellD
  • NRG

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