Thank you thank you very much oh youre perfectly

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Thank you. Thank you very much.” Oh you're perfectly welcome, sir. © 1980 LucasFilm Ltd.
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Lab 2 Page 12 Troubleshooting Streaking The Point of Streaking is to get isolated Colonies. If you have any isolated Colonies on your Plates -- regardless of where they ʼ re located -- you have a useable Streak Plate. And a useable Streak Plate is a good Streak Plate. Ideally, you ʼ d like to get lots of isolated Colonies in the “C” Section. But a lot of Microbiologists -- including me -- usually get very few or no Colonies in the “C” Section, but lots of Colonies in the “B” Section. That ʼ s OK. Here are some commonly encountered Problems: • No Colonies whatsoever in any Section - Your Loop was too Hot. When you sterilize your Loop you’re heating it up to ~1100°C so be sure it’s cooled-down before you try and pick-up any Bacteria. Let your Loop cool in the Air for a few Seconds and then cool your Loop in the Agar after you’ve sterilized it. - You didn’t pick-up any Bacteria in the first Place. This is particularly easy to do with Bacteria like Staphylococcus growing on YGC Agar because you can mistake the Zone of Clearing for the Edge of the Colony. • Lots and lots of Bacteria in all three Sections (A, B and C) - You forgot to sterilize your Loop between streaking each Segment. - You streaked your Loop repeatedly through the previous Section and picked-up way too many Bacteria • Lots and lots and lots of Bacteria in the “A” Section but none in any other Section - You didn’t streak your Loop far enough into the “A” Section and didn’t pick-up any Bacteria. - Your Loop was too Hot and you fried the Bacteria you picked-up. • The Colonies are unusually small - You used the wrong Medium - You put your Streak Plates in the wrong Bin and they were incubated at too low a Temperature. • The Colonies are a different Color or Morphology from the original Plate. - You picked-up from a contaminating Colony on the original Plate - You left the Lid off for too long and contaminating Bacteria in the Air fell onto the Plate (check if the Colonies are on a Streak Line).
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Lab 2 Page 13 Pseudomonas (Selection) Do not vortex your Pseudomonas Test Tube. Pseudomonas is motile and you ʼ re using its Motility to isolate it from all the other Bacteria in the Soil that aren ʼ t motile. These other Bacteria are in the Bottom of your Test Tube and you want them to stay there. That ʼ s why you don ʼ t want to vortex your Pseudomonas Test Tube. 1. If the Sodium Benzoate Medium in the Test Tube you inoculated with a Pinch of Soil is Turbid, use your Sterile Inoculating Loop to transfer a Loopful of this Medium to do the Primary Streak on a (labeled!) Sodium Benzoate Plate (Green Stripe). 2. Re-Sterilize your Loop and perform the Secondary and Tertiary Streaks as usual. 3. If the Sodium Benzoate Medium in your Tube is not Turbid, either proceed on Faith or take a Loopful of Medium from someone else’s Tube that is Turbid.
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