Group Members Ben Kensing Nicole Tran Kevin Yee Clark Plost Exercise2Date 13014

Group members ben kensing nicole tran kevin yee clark

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Group Members: Ben Kensing, Nicole Tran, Kevin Yee, Clark Plost _____Exercise___2___Date: 1/30/14______ LI ______________________ Unique 49200 Day: Wednesday____ Room: PAI 1.24 Lab Start Time 6 (3)INDIVIDUAL:Paramecia(permanent dry and fresh wet mounts) (a)Based on your experience in this exercise, report in tabular form at least two advantages and two disadvantages of permanent dry mounts compared with wet mounts for determining features of specimens as observed by brightfield microscopy. Objective MagnificationObjective NaOcular MagTotal MagResolution “d” (μm){λ==??? nm}Reticle units/ 0.1mmmm/r.uμm / r.u.4X0.110X40X3.05040.0252510X0.2510X100X1.220100.011040X0.6510X400X0.465231400.00252.5100X1.2510X1000X0.2441000.0011Exercise 1 Page of 46Dry MountsWet MountsAdvantagesEasier to LocateCan Dye the specimen without harming itSet number of specimens Live specimensBetter at seeing how the cell movesActual size of celldisadvantages Dead SpecimensUnable to see cell size when its aliveUnreal colorHarder to findUnable to use dyes that could kill the specimenClear cell like in the picture below
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Group Members: Ben Kensing, Nicole Tran, Kevin Yee, Clark Plost _____Exercise___2___Date: 1/30/14______ LI ______________________ Unique 49200 Day: Wednesday____ Room: PAI 1.24 Lab Start Time 6 (b)Include a digital image of Parameciapresent in a permanent dry as well as a fresh wet mount. (c)Based on the length measurements, do the Parameciaused for the permanent dry mount and the sample of Parameciaused for the wet mount likely belong to the same species? State the null hypothesis concerning these sets of measurements. Use the class data set to perform a t-test and support your conclusion when explaining your answer. Provide the means, standard deviations (including units) and p-values. Interpret the results. (i)If the p-value indicates a statistically significant difference: Can you reject the null hypothesis and infer that the two sets of measurements reflect a real and meaningful difference? Can it be concluded that these two sets of Parameciareally belong to two different species? Suggest an explanation for this difference. (ii)If the p-value indicates a lack of a statistical difference: Does this indicate that the null hypothesis has been confirmed? Explain your answer, taking into account sample sizes, experimental design (e.g., wet vs.dry specimens), and possible confounding factors. The null hypothesis between the dry mount and wet mount of Paramecia is that if the two data sets from the lengths of each mount are similar then the species from the wet mount and dry mount are the same species. The mean length and standard deviation of the dry mount Paramecia was 158.804 μm and 38.360 μm receptively. The mean length and standard deviation of the wet mount Paramecia was 192.61 μm and 24.72 μm receptively. The p- value from the t-test is .001047803. From the p-value, which is less than .01, there is a significant difference between the mean of both the wet and dry mount. This would conclude that the two specimens are from two species. However, the p-value could be inaccurate due to human error in measurement or converting r.u. to μm. The p-value can also compromised by the fact that the dry mount of the Paramecia were dead and dyed for better contrast. This dye or the fact that the cell membrane and other organelles are no longer functional can affect the relative size of the cell. I would make sense that the cell would decrease in size after dying, which is shown in the data. Exercise 1 Page of 56
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Group Members: Ben Kensing, Nicole Tran, Kevin Yee, Clark Plost _____Exercise___2___Date: 1/30/14______ LI ______________________ Unique 49200 Day: Wednesday____ Room: PAI 1.24 Lab Start Time 6 Individual: Discuss the demonstration material.(a)Submit several digital images and/or sketches of specimens you observed in the lab. (b)Based on your own observations in the lab, which external and internal cellular structures do the organisms appear share? Which cellular structures appear unique?
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  • Spring '08
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  • Statistics, Statistical hypothesis testing, Statistical significance, Aperture, Clark Plost _____Exercise___2___Date

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