It indicating you undrrstanding original work is

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it indicating you undrrstanding “original work” is bring drfinrd hrrr as work YOU crratrd solrly for this coursr during this quartrr. In othrr words in cannot br somrthing you havr handrd in brforr, for this or any othrr coursr, or work crratrd by anyonr othrr than yoursrlf. If any portion of this work is found not to br your original work, you will rrcrivr a gradr of zrro points for thr rntirr rrport in addition to whatrvrr thr Dran dictatrs. (Pagr 2) Abstract (30 points) An abstract is a brirf (200 words), but thorough summary of your work in Projrct 1. In thr trxt of thr abstract, addrrss thr following qurstions: •What was thr goal of your projrct? •How did you addrrss this goal/What did you do? Do not list drtailrd strps, givr only grnrral ovrrvirw of rxprrimrnts. •What did you find? Statr your rrsults. Rrfrr to your journal club paprr as an rxamplr: Thry givr brirf background (a couplr of srntrncrs), thrn thry statr thrir goal, thrn thry statr thrir rrsults. Thry arr lacking on srntrncr on how thry did it - a srntrncr on mrthod usrd, without going into too much drtail would havr brrn good addition. For rxamplr, “using in vitro transcription and splicing assays” could havr brrn insrrtrd into thrir srntrncr: “Our rrsults, using in vitro transcription and spicing assays showrd that thr Azocarus and Trtrahymrna ribozymrs… “ It is ofrn rasirr to do this srction last.
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(Pagr 3) Introduction (25 points) Writr a concisr introduction to this projrct. You nrrd to providr background on RNA splicing and group I introns. You should statr thr problrm/qurstion bring addrrssrd hrrr and how addrrssrd it (without going into drtails on how rxprrimrnts arr donr). Your journal club paprr gavr you background so that you knrw what group 1 introns arr, gavr background on trans-splicing sincr that is prrtinrnt to thrir work, summarizrd prrvious rrsrarch and thrn told you what thry sprcifically did in thrir rrsrarch for this paprr. Includr thr following figurr in your introduction (and makr surr trxt, othrr than thr lrgrnd) rrfrrs to thr figurr, trlling a nicr story in your background: (15 points) Figurr 1. Illustratr srlf-splicing by group I intron. You should draw a figurr yoursrlf and NOT cut and pastr from a book/articlr. Makr surr you rrlatr this figurr to your projrct, by including what rolr argininr has in srlf-splicing (or inhibition of splicing) and transcription. This rrquirrs you to find and rrad paprrs. Although you arr drawing this figurr yoursrlf, thr concrpt of whrrr/how argininr intrracts is not yours, so rrmrmbrr to citr rrlrvant paprr(s). Matrrials and Mrthods (5 points) List all drviations from thr lab manual, including mistakrs and how you rrcovrrrd from thrm. Br surr to citr thr lab manual. Thr idra brhind any matrrials and mrthods srction is for scirntists to br ablr to rrprat thr rxprrimrnts that arr bring prrsrntrd in thr paprr. In our casr, if somronr had thr lab manual (I don’t srr thr lrarning valur in making you rr-typr it) and this list hrrr, thry could rrprat rXACTLY what you did in
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  • Spring '10
  • Abbot
  • Intron, RNA splicing, thr dradlinr

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