15 sf plate a strepto faecalis differentiates

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15. SF plate a. Strepto Faecalis differentiates Enterococci from Strept bovis + other b. Recommended for examination of water and pollution c. Enterococci ferment dextrose and grow in presence of inhibitor sodium azide i. Peptone and dextrose supply nutrient for growth ii. NaCl maintains osmotic balance iii. Na Azide – bacteriostatic on g- bact iv. Bromcresol purple indictor >>>>> Purple: -
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v. Yellow and turbit: + 1. Blood agar selects for G+ bact and can differentiate among them based on hemolytic rxn 2.Bile esculin agar selects for group D stepto and enteroc and is differential cased on the ability to hydrolyze esculin in the presence of bile 3. Most staph can grow on Mannitol salts agar but very few, like Staph aureus, can ferment the mannitol – TRUE 4. MacConkey Agar has selective agents that inhibit the growth of G+ and is differential based on the ability to ferment lactose 5. On the EMS agar, nonfermentators of lactose will appear CLEAR – TRUE 6. Bacteriostatic agents DO NOT kill most or all bacteria. Bacteriocidal DO! 7. Natural agents used to treat bact infectins, produced by microorganisms are called Antibiotics 8. S salivarius growing on Mitis Salivarious Agar would appear… 1-5mm large blue gum drop colonies 9. SF Media is used to detect the presence of Fecal Streptococci 10. A DISINFECTANT is used on surfaces LAB 10 1. Yogurt Production a. Lactobacillus bulgaricus and Streptococcus thermophiles i. Both contain B-galactosidase that break down lactose and convert glucose to lactase, formate and acetate (via pyruvate in glycolysis) b. Yogurt gets its flavor from acetaldehyde, diacetyl, and acetate produced from the fermentation of lactose i. Depends on proportions of products ii. Sweetness is results of accumulation of galactose 1. Stept thermos cant break it down 2. Lacto bulg. Uses glucose first 2. Closes sytem Growth a. Lag- Adjustment period of the cells, no initial significant growth b. Exponential- Very rapid cell division (2x), using up nutrients rapidly c. Stationary- Nutrients running out along with toxins building up. Death rate = Growth rate d.
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  • Summer '08
  • FRYE
  • Bacteria, Growth medium, i., Mitis Salivarius Agar, Endo agar coliforms

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