UCSB_MCDB110_FinalExam_Winter2009-1497285260178.pdf

The key conclusion from this experiment was that a

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The key conclusion from this experiment was that: a) After being denatured, all proteins require chaperonins to renature to an active form. b) All proteins contain disulfide bonds that stabilize their 3-D structure. c) The information for the proper 3-D folding of a protein is contained within the primary amino acid sequence. d) Hydrophobic interactions cannot influence protein structure. e) Ribonuclease cannot be subject to irreversible inhibition.
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Name ____________________ -13- 46. Which of the following types of reaction does NOT occur in fatty acid catabolism? a) isomerization b) hydration c) dehydrogenation d) thiolytic cleavage e) electron transfer reaction 47. The following is NOT a part of secondary active transport a) transport driven directly by an ion gradient across the membrane b) transport driven directly by ATP hydrolysis c) symporter d) antiporter e) none of the above 48. Which of the following is the most efficient molecule used by cells to store energy? a) proteins b) sphingolipids c) carbohydrates d) triacylglycerols e) cholesterol 49. (4 points). Explain why, although it involves synthesis of ATP from ADP, the last step of glycolysis is highly exergonic. What is it about the metabolites in the reaction that accounts for the very favorable free energy? The reaction is: PEP + ADP Pyruvate + ATP Immediately upon transfer of its Pi to ADP, pyruvate is generated in the enol form, which is unstable and spontaneously tautomerizes to the more stable keto form. This tautomerization strongly drives the reaction forward (i.e., makes it highly exergonic). 50. (6 points). Name three specific factors that affect membrane fluidity and describe in which way they affect it (increase or decrease). Heat: increases, cooling decreases fluidity Increased saturation results in decreased fluidity (and vice-versa ) Increased fatty acid tail length results in decreased fluidity (and vice-versa ) Sterols: at high temp decrease fluidity, at low temp increase fluidity
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Name ____________________ -14- 51. (13 points). Answer the following questions for this compound: a) (3 points). This compound is called _ coenzyme A (CoA) ___ (full credit only for the correct number of words). (It is not acetyl-CoA) b) (4 points). Circle and label the “business end” of the molecule. Describe the type of reactions that this molecule participates in (how does this molecule do its chemistry?). CoA carries acyl metabolites, for example, acetyl and succinyl groups, through a high-energy thioester bond involving the terminal sulfur atom. c) (3 points). Two pathways or reactions in which the molecule participates are: eta-oxidation of fatty acids, pyruvate dehydrogenase reaction, succinyl coA synthetase reaction (TCA cycle) d) (3 points). Included in the molecule is another important structure we have studied called _ ADP ___________. Circle and label that part of the molecule.
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