In a fluorescent microscope which of the following

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Chapter 7 / Exercise 12
A+ Guide to Hardware
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14. In a fluorescent microscope, which of the following reflects light of a given set of wavelengths, and allows full transmission (no reflection) of light of a different set of wavelengths? (A) excitation filter (B) dichroic mirror (C) emission filter (D) objective lens (E) half-set filtered objective drive
15. Photoactivation microscopy involves which of the following:
16. Which of the following microscopy methods would be able to resolve two distinct fluorescent objects of 20 nm, located 100nm apart?
17. Which of the following pairs of techniques and labeling methods would be useful when used together?
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A+ Guide to Hardware
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Chapter 7 / Exercise 12
A+ Guide to Hardware
Andrews/Dark
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5 18. Which of the following is able to intercalate within a lipid bilayer (e.g. the plasma membrane) and allow visualization of these organelles by fluorescence microscopy? (A) all secondary antibodies (B) quantum dots (C) fluorescein (D) Gold-particle conjugated antibody (E) FM4-64
19. Which of the following is the best explanation of how deconvolution microscopy allows removal of out-of-focus light?

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