Contacts cytoplasmic actin and unc 84 unc84 likely

Info icon This preview shows pages 23–33. Sign up to view the full content.

View Full Document Right Arrow Icon
contacts cytoplasmic actin and UNC-84 . UNC84 likely interacts indirectly to lamins; not known if UNC84 is an INM and/or a ONM protein. UNC84 through UNC83 binds to microtubules, required for association of centrosome and the nucleus, through a end directed motor. Gruenbaum et al (2005) Nature Reviews Mol Cell Biol 6, 21-31.
Image of page 23

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Organisation of the Nuclear Envelope from Burke and Stewart (2002) Nature Reviews Mol Cell Biol. 3, 575. b | Overview of NE organization and the relationship of the nuclear membranes to the endoplasmic reticulum (ER). In addition to the main structural features, including the ONM, INM, nuclear pore complexes (NPCs) and lamina, some INM and chromatin proteins are also indicated. BAF, barrier to autointegration factor; LAP, lamina- associated polypeptide; LBR, lamin B receptor.
Image of page 24
Inner Nuclear Membrane Proteins Examples: Lap2 β , Lap2 γ , emerin, MAN1, lamin B receptor (LBR) Large nucleoplasmic domains that associate with chromatin and nuclear factors, many have a LEM domain (43-residue sequence), bind to Lamin A and B, and BAF (a DNA- binding protein that may link the NE to chromatin). LEM proteins and BAF required to work with lamins to assemble the NE (post-mitotic event). Lamins and LEMs are important for gene regulation Several transcription factors bind lamins e.g. RB (transcription factor that regulates entry into S-phase).
Image of page 25

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
LBR links lamina to HP1 (Heterochromatin Protein1), a component of silenced chromatin. LBR may be part of the mechanism for positioning heterochromatin at the NE. Gruenbaum et al (2005) Nature Reviews Mol Cell Biol 6, 21-31.
Image of page 26
The Nucleolus: an example of a sub-nuclear compartment Nucleolus structure and ribosomal gene transcription Established as the site of ribosomal biogenesis by Vincent and Miller in 1966 Structural appearance of nucleolus is related to transcriptional activity of ribosomal RNA genes, disassembles in mitosis. 200 copies of the ribosomal gene are found in clusters on 5 chromosomes (human). 10 chromosomal regions (diploid) coalesce to form the nucleolus. Integrity is lost when cells exposed to inhibitors of RNA polymerase I
Image of page 27

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
In humans, Nucleolar Organizing Regions (NORs) contains genes for 5.8S, 18S, and 28S rRNA . These are clustered on the short arms of 5 chromosomes; 13, 14, 15, 21 and 22.
Image of page 28
Functional domains within nucleolus (rDNA) (Pre-ribosomal particles) (Maturation of pre-rRNA Transcripts)
Image of page 29

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Nuclear Speckles- another possible gene “anchoring” site SC35-- a splicing factor found in Nuclear Speckles PML Nuclear Bodies DAPI
Image of page 30
COL1A1 and COL1A2 genes and transcripts associate with a single, common domain Shopland et al. (2003) J Cell Biol 162, 981 Genes RNA Transcripts Deconvolution SC35 (Ab) SC35 (Ab) DNA or RNA detected by fluorescence in situ hybridization Transcripts Gene
Image of page 31

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
( a ) RNA immuno-FISH of Hbb-b1 transcription (red) with RNAP II staining (green) in anemic spleen erythroid cells.
Image of page 32
Image of page 33
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern