Solution Hybridization neither the probe nor the target is immobilized o Target

Solution hybridization neither the probe nor the

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Solution Hybridization – neither the probe nor the target is immobilized o Target bind solution followed by resolution of the bound products
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Chapter 7: Nucleic Acid Amplification Target Amplification – Making copies of a target sequence to such a level that they will be detected in vitro Primer Dimers – PCR products that are just double the size of the primers Thermal Cyclers – designed to rapidly and automatically ramped to the required incubation temperatures holding at each one for designated periods Rapid PCR – work with small sample volumes in chambers that can be heated and cooled quickly by changing the air temperature surrounding the samples Real-Time PCR – equipped with fluorescent detectors to measure PCR product as the reaction proceeds. Pre-PCR – preparation of the specimen for PCR is often referred Contamination control/Reagent blank – ensures that reaction mix is not contaminated with template DNA or amplified products from previous run Psoralens – effectiveness of uv light may be increased Multi-plex PCR- useful in typing or identification analyses Quantitative PCR – performed by adding ethidium bromide to a regular PCR Molecular Beacons – measures the accumulation of product at the annealing step in the PCR cycle Scorpion – variation of molecular beacons Fluorescent Resonance energy transfer – Utilizes two specific probes on with a 3’ fluorophore acceptor and the other with a 5’catalyst for the fluorescence donor. Arbitrarily primed PCR – random amplification of polymorphic DNA short primers with random sequences are used to amplify arbitrary regions in genomic DNA under low stringency conditions Comparative genomic arrays – detection of single nucleotide polymorphisms and analysis of minimal starting material such as DNA Plasma Branched DNA amplification – series of short oligomer probes are used to capture the target nucleic acid. Cleavage-based amplification – detects target nucleic acids by using a series of prboes that bind to the target and overlap Cycling Probe – target sequences are detected using a synthetic probe consisting of sequences od DNA and RNA arranged in a DNA-RNA-DNA sandwich sequence carrying a reporter dry at one end and quencher dye.
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  • Spring '19
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