Thats no moon its a space station 1977 lucasfilm ltd

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___________________________________ That's no moon. It's a space station. © 1977 LucasFilm Ltd.
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Lab 1 Page 25 3. Purification An Isolated Colony on a Benzoate Plate will be used to streak a Nutrient Agar Plate, which will then be incubated at 30°C for at least 48 Hours. A Gram Stain of Bacteria from an Isolated Colony should reveal Gram Negative Rods. A Wet Mount of Bacteria from an Isolated Colony should reveal Motile Rods. 4. Identification Isolated Colonies on a Nutrient Agar Plate will be used for Biochemical Identification Tests. These include the Oxidase Test and a special F Medium (F for Fluorescence) will be streaked to check for characteristic Fluorescence. Biohazard Note You are performing an Isolation of an unknown Strain of Pseudomonas . The Strains of Pseudomonas you will isolate are almost certainly Benign, but they should be handled as if they are Pathogenic. Some Strains of Pseudomonas can be Opportunistic Pathogens (particularly in Association with serious Burns). ________________________________________________________ “Garbage chute. Really wonderful idea. What an incredible smell you've discovered! Let's get out of here! Get away from there... “ No, wait...!Will you forget it? I already tried it. It's magnetically sealed!’ Put that thing away, you're gonna get us all killed! Absolutely, Your Worship. Look, I had everything under control until you led us down here! Now it's not going to take them long to figure out what happened to us.” It could be worse. It's worse.” © 1977 LucasFilm Ltd.
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Lab 1 Page 26 Gameplan for the Isolation of Bacillus sp. The Genus Bacillus contains Gram Positive, Spore-Forming Rods, often Motile by Peritrichous (G= hairs around) Flagella. They are Facultative Aerobes. Bacillus represents a significant Portion of the Soil Microbial Population so we’ll be able to utilize Direct Isolation as our First Step. Since Bacillus is a Spore-former, and since Spores are highly resistant to Heat, a simple Pasteurization Step can be used to further increase the relative Representation of Bacillus in a Soil Sample. Most Soil Bacteria cannot survive heating to 80°C. Their Proteins are denatured at this Temperature. But the Proteins in Bacterial Spores do not denature at this Temperature (probably due to the near Total Elimination of Water Content during Spore Formation). There are two commonly encountered Spore-Forming Bacteria in Soil Samples: Bacillus and Clostridium . The Genus Clostridium includes the Causative Agents of Botulism, Gangrene, and Tetanus. ¡Canasta! We don’t want to mess with any of those Diseases! But Clostridium Species are Obligate Anaerobes (they cannot tolerate Oxygen). Bacillus can be separated from Clostridium by Incubation under Aerobic Conditions. Bacillus is the only Spore-Forming Species that can grow under our Aerobic Incubation Conditions.
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