45 triple sugar iron agar ingredients gl peptic

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45.Triple Sugar Iron Agar Ingredients g/l Peptic digest of animal tissue : 20.0 Meat extract : 3.0 Yeast extract : 3.0 Lactose : 10.0 Sucrose :10.0 Glucose : 1.0 Ferric citrate : 0.3 Sodium chloride : 5.0 Sodium thiosulphate, pentahydrate : 0.3
WATER ANALYSIS 2015 282 Phenol red : 0.024 Agar : 12.0 Final pH (at 25°C) 7.4±0.2 Directions:Suspend above ingredients in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Adjust the pH and distribute into test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the mediumto set in sloped form with a butt about 1 inch long.
WATER ANALYSIS 2015 283 46.Tributyrin Agar Base w/o Tributyrin Ingredients g/l Peptic digest of animal tissue : 5.0 Yeast extract : 3.0 Agar : 15.0 Final pH ( at 25°C) 7.5±0.2 Directions:Suspend above ingredients in 1000 ml distilled water. Add 2g of Tributyrin. Heat if necessary to dissolve the medium completely. Adjust the pH and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Shake the flask and individual plate so as to maintain uniform turbidity.47.Tryticase-soy-sodium Chloride Broth Ingredients g/l Tryptone : 15.0 Phytone : 5.0 Dipotassium Phosphate : 2.5 Sodium Chloride : 30.0 Glucose : 2.5 Final pH ( at 25°C) 7.3±0.2 Directions:Suspend above ingredients in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Adjust the pH and dispense into sterilized test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.48.Tryptone Broth Ingredients g/l Tryptone : 10.0 Final pH ( at 25°C) 7.5±0.2
WATER ANALYSIS 2015 284 Directions: Suspend 10 g of tryptone in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Adjust the pH and dispense into tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
WATER ANALYSIS 2015 285 49.Tryptophan Medium Ingredients g/l Casein enzymic hydrolysate : 10.0 Sodium chloride : 5.0 DL-Tryptophan : 1.0 Final pH (at 25°C) 7.5±0.1 Directions: Suspend above ingredients in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Adjust the pH and dispense into tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. 50.Urea Agar Base, Christensen Ingredients g/l Peptic digest of animal tissue : 1.0 Dextrose : 1.0 Sodium chloride : 5.0 Monopotassium phosphate : 2.0 Phenol red : 0.012 Agar : 15.0 Final pH ( at 25°C) 6.8±0.1 Directions:Suspend above ingredients in 950 ml distilled water. Heat if necessary to dissolve the medium completely. Adjust the pH and sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes. Cool to 50°C and aseptically add 50 ml of sterile 40% Urea Solution and mix well. Dispense into sterile tubes and allow to set in a slanting position. Do not overheat or reheat the medium as urea decomposes very easily.
WATER ANALYSIS 2015 286 51.Wagatsuma Agar Base Ingredients g/l Peptic digest of animal tissue : 10.0 Yeast extract : 3.0 Sodium chloride : 70.0 Dipotassium phosphate : 5.0 Mannitol : 10.0 Crystal violet : 0.001 Agar : 15.0 Final pH ( at 25°C) 8.0±0.2 Directions: Suspend above ingredients in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Adjust the pH and steam for 30 min and cool to 50°C. Add 100 ml of 20% suspension of fresly drawn citrated human red cells (previously washed three times in sterilized physiological saline). Mix well and pour into sterile petridishes

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