Immunology Lecture Exam 3 - Study Guide Chapter 5 & 6 - Kara.docx

Which chain rearranges vdj and which chain rearrange

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40. Which chain rearranges VDJ and which chain rearrange VJ only? Which are rearranged first, heavy, light, D-J or V-D? There are quite a few ways I could ask these questions, look at these topics from different angles and make sure you know it well. a. Heavy chain is first, (D to J, DJ to V,) then light chain (V to J) b. Early pro-B= Heavy D-J rearrangement, Light germline c. Late pro-B= Heavy VDJ rearrangement, Light germline d. Large pre-B= Heavy VDJ rearrangement, Light germline e. Small pre-B= Heavy rearrangement, Light VJ rearrangement (no longer have pre-B cell receptor; Ig μ heavy chains restricted to cytoplasm; rearrange of Ig Light-chain loci begins) Knight Essentials
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Kara Kohlmaier f. Immature B-cell= Heavy rearrangement, Light VJ rearranged and has not yet met antigen, has only IgM on surface g. Mature B-cell= Heavy and Light rearranged, has both IgM & IgD on surface, can also be naive 41. Surrogate light chain, l5 and VpreB. What each subunit substitutes for, when and where is this happening? Why do you need this subunit produced? Is this one of the checkpoints during maturation?: marrow in large Pre-B cells work with light chain 42. Iga and Igb, what do these do and when would you expect to see them expressed? a. Large pre-B cells b. Help BCR signaling, and see them anytime you have a membrane bound BCR c. They help surrogate light chain to form a complex resembling the B cell receptor 43. Immature B cell vs. mature B cell, are there differences? a. Immature B-cell = only has IgM on the surface b. Mature B-cell = has both IgM and IgD on surface 44. Large Pre-B cell → Small Pre-B cell, what significant happens or is formed at this point in B cell maturation? 45. Anergy vs. clonal deletion: a. Anergy: when cell becomes inactivated and unresponsive to their specific antigen, said to begin developmental arrest Knight Essentials
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Kara Kohlmaier b. Clonal Deletion: is the selective death of lymphocytes that are either autoreactive (light chain rearrangements fail to produce non-self reactive receptors) or fail to make a functional immunoglobulin 46. Development of B cells, where and steps and names.: a. Stem Cell → Early pro-B Cell (D-J rearrangement) → Late pro-B Cell (D-V-J rearrangement) → Large pre-B Cell (functional heavy chain occurs and proliferation of B cell into about 100 progeny occurs. The heavy chain is expressed on the surface of the cell along with a surrogate light chain that is made up of VpreB [variable region] and lambda 5 [constant region] as a Pre-BCR. This allows the B cell to test to make sure the heavy chain is functional) → Small pre-B cell (light chain V-J rearrangement occurs and functional heavy and light chain are presented on surface as BCR) → Immature B Cell (Immature meaning that only Igm is present on cell
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  • Winter '17
  • T Cell, Major histocompatibility complex, B cell, Kara Kohlmaier

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