Lab__1b_Viewing_Bacteria_with_Microscope

Do not remove agar with your sample sterilize

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TSY agar. DO NOT remove agar with your sample!! * sterilize inoculation loop in microincinerator and let cool * 5. Using inoculation loop, obtain a small sample of Escherichia coli (E. coli) from the source plate or tube. Swirl loop in circle of water in the middle. * sterilize inoculation loop in microincinerator and let cool * 6. Using inoculation loop, obtain a small sample of Bacillis subtilis from the source plate or tube. Swirl loop in circle of water on right. * sterilize inoculation loop in microincinerator and let cool * 7. Heat fix the slide on top of your microincinerator . Allow it to stay in the platform for 5 minutes after water has completely evaporated. This kills the bacteria and sticks it to the slide. 8. Stain the slide with crystal violet for 45 seconds then observe under oil immersion . Draw circles with wax pencil Put a drop of water in each circle. Inoculate circle of water with each of the following: 1 . Staph epi 2 . E. coli , 3. Bacillus subtilis . After heat fixing on microincinerator, stain with crystal violet, rinse, then look at with scope. 1. 3. 4 - 6 8. From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Although you will be using 100 xTM to get your specimen in focus, you will not be able to see individual bacteria until you use the 1000 xTM oil immersion lens. Bacterial smear at 100xTM Bacterial smear at 1000xTM (oil immersio n) FYI: The example above is Staph . Images: Both images Staphylococcus , by T. Port Remember, you want to use the microscope with a built in camera so that you can take pictures of the bacteria you see at 1000xTM.
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  • Fall '02
  • Liao
  • Bacteria, pH, Telescope, Virtual Microbiology Classroom, T. Port

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