Lab__1b_Viewing_Bacteria_with_Microscope

Important very gently obtain sample of bacteria from

Info iconThis preview shows pages 7–9. Sign up to view the full content.

View Full Document Right Arrow Icon
IMPORTANT!!!: Very gently obtain sample of bacteria from the source plate or tube. The bacterial colonies are found growing on the surface of the TSY agar. DO NOT remove agar with your sample!! * sterilize inoculation loop in microincinerator and let cool * 5. Using inoculation loop, obtain a small sample of Escherichia coli (E. coli) from the source plate or tube. Swirl loop in circle of water in the middle. * sterilize inoculation loop in microincinerator and let cool * 6. Using inoculation loop, obtain a small sample of Bacillis subtilis from the source plate or tube. Swirl loop in circle of water on right. * sterilize inoculation loop in microincinerator and let cool * 7. Heat fix the slide on top of your microincinerator . Allow it to stay in the platform for 5 minutes after water has completely evaporated. This kills the bacteria and sticks it to the slide. 8. Stain the slide with crystal violet for 45 seconds then observe under oil immersion . Draw circles with wax pencil Put a drop of water in each circle. Inoculate circle of water with each of the following: 1 . Staph epi 2 . E. coli , 3. Bacillus subtilis . After heat fixing on microincinerator, stain with crystal violet, rinse, then look at with scope. 1. 3. 4 - 6 8. From the Virtual Microbiology Classroom on ScienceProfOnline.com
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Although you will be using 100 xTM to get your specimen in focus, you will not be able to see individual bacteria until you use the 1000 xTM oil immersion lens. Bacterial smear at
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Page7 / 10

IMPORTANT Very gently obtain sample of bacteria from the...

This preview shows document pages 7 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online