02 This could be caused if not the compound in low concentration was spotted

02 this could be caused if not the compound in low

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versus when both compounds were separated together, with a difference of only 0.02. This could be caused if not the compound in low concentration was spotted when both compounds underwent chromatography together or if the distance was not measured properly. In part b, unknown #1 was determined to be Tylenol. During the chromatography, the unknown compound moved almost the same amount of distance as Acetaminophen. Even though there were other spots on the chromatography paper, they were not significant enough to identify them as a compound. Since the only brand with Acetaminophen as its sole ingredient is Tylenol, it is safe to say that the unknown compound is Tylenol. The small insignificant spots could have been caused if any organic compound were accidentally spilled on the TLC plate or the surface of the plate was touched with hands. Conclusion The Rf values of o -hydroxyacetophenone and p -hydroxyacetophenone were 0.37 and 0.27 respectively when the chromatography was done separately. When both compounds were separated together, the Rf values were 0.25 and 0.35. Since the Rf values were so close, it was determined that the Rf value of 0.35 was referring to o -hydroxyacetophenone and 0.25 was
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  • Fall '13
  • JeramyBaum

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