The experiments that cleared up this situation and

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import.  The experiments that cleared up this situation- and helped define our present  view of nuclear import-showed that some Ran-GEF leaks out of nuclei during preparation of cell remnants.   (A)  How might the presence of Ran-GEF outside the nucleus lead to a spurious  requirement for GTP hydrolysis in nuclear import?   -Presence of Ran-GEF outside of the nucleus leads to a spurious requirement for  GTP hydrolysis because GEF’s activate a GTPase since GEF’s favor the GTP form. It is  safe to assume that because Ran-GEF increases the rate of GDP to GTP exchange  causing there to be a high concentration of GTP in the cytoplasm allowing for  hydrolyzation. It is understandable why people would believe that GTP hydrolysis is the  essential for protein import into the nucleus. (B)  Why does a nonhydrolyzable analog of GTP such as GppNp block import under  these conditions while GTP does not? - Nonhydrolyzable analog of GTP, GppNp, is able to block the import of protein  because in order for importing to grab a cargo protein, they must be released from GTP  by hydrolyzation. If the GppNp is unable to be hydrolyzed, then the importin won’t be  released and wont be able to bind to the cargo protein, thus blocking import. GTP wont 
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