Orange mt concentrated in the central domain

This preview shows 9 out of 16 pages.

Orange, MT concentrated in the central domain
Image of page 9

Subscribe to view the full document.

Microfilaments (MF) Actin filaments F-actin – Filaments composed of globular actin – ATP – flexible – cell shape, locomotion – Most eukaryotes, some bacteria – 8nm diameter helical filament – Myosin motors – 20% of muscle protein. 5-10% of non-muscle protein
Image of page 10
Microtubules (MT) – filaments, composed of globular tubulin – alpha and beta – Stiff, hollow rods – GTP – position of membrane organelles, intracellular transport – All eukaryotes, some bacteria – Composed of alpha and beta tubulin – 25nm in diamter – Kinesin and dynein motors
Image of page 11

Subscribe to view the full document.

Intermediate filaments – composed of elongated subunits – mechanical strength, resistance to shear stress – Rope-like – Limited to higher eucaryotes – Diverse proteins encode these type of filaments – 10 nm diameter filament: hence “intermediate
Image of page 12
Image of page 13

Subscribe to view the full document.

What technique was originally used to define these filaments? What techniques would you use to observe dynamic feature of cytoskeleton filaments?
Image of page 14
Filament Dynamics Two ways to generate force Motor proteins Filament dynamic Time course of polymerization in a test tube -- Rate constants (depend on temp, salt) k on = rate constant for the addition of one subunit to a polymer k off = rate constant for the loss of one subunit from a polymer k on is dependent on the concentration of free subunits ( C ). So k on C k off does not depend on C C c = Critical Concentration is when the concentration of free subunits drops until the on and off rates are the same: k on C = k off C c = k off / k on
Image of page 15

Subscribe to view the full document.

Image of page 16
You've reached the end of this preview.
  • Spring '08
  • Scholey
  • molecular biology, Garland Science, Molecular Biology of the Cell

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern