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L.05.Bacteriophage

Lyses bacteria does not lyse bacteriophage the

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• Lyses Bacteria • Does not lyse Bacteriophage The Doermann Experiment involves artificially Lysing the Infected Bacteria at each Ellis-Delbrück Experiment Timepoint. - The resultant Intracellular Growth Curve gives you an “Inside the Bacterium” View of the Phage Growth Kinetics. Think of the Doermann Experiment as an early Attempt to open a “Doer” into the Latent Period. The Intracellular Growth Curve • Eclipse Period (Minute 0-12) - The Time during which no Phage whatsoever can be detected by Artificial Lysis - The Phage has disappeared (!) • Maturation Period (Minute 12-36) - Artificial Lysis (and now Natural Lysis) is releasing increasing Numbers of new Progeny Phage • Plateau Period (Minute 36+) - All Infected Bacteria have lysed (Artificial and Natural Lysis) - All Progeny Phage have been Released • The Doermann Experiment indicated that Bacteriophage were being assembled during the Second Half of the Latent Period - So the Question again shifted: • What was going on during the Eclipse Period? And so Molecular Biologists once again turned to that critically important, incredibly sophisticated Molecular Biological Tool -- The Waring Blender...
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Bacteriophage Page 4 1952 The Waring Blender Experiment The Waring Blender -- invented by the Band Leader Fred Waring for After-Gig Drinks -- was an important Technological Development for Molecular Biology. The Waring Blender Experiment was a dirty but convincing Experiment that utilized an imaginative Combination of Radioisotopes, Bacteriophage and a Waring Blender. Bacteriophage were known to be composed of DNA and Protein. And Early EM Studies implied that these Phage attached to the Bacterium and injected something into the Bacterium. This “something” contained all the necessary Information for making more Phage. Phage were labeled by growing them in Bacteria that had been maintained in Radioisotopes 35 S 32 P These Phage would These Phage would have labeled Protein have labeled DNA - Infect Batches of Bacteria with these different Phage • Allow only enough Time for the Phage to attach to the Bacteria and inject their “something” - Dump Bacteria with attached Phage into a Blender and turn it on.
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