Value is the initial molarity of the reagents m 2 is

This preview shows page 3 - 6 out of 16 pages.

value is the initial molarity of the reagents, M2 is the final molarity of the concentration made, V1is the volume of the original substance to create the desired molarity, and V2is how much solvent should be added to make a solution of a standard size. Once the standard concentrations have been calculated, make them with one dye at a time. Prepare a 10mL sample for each of the five concentrations, in the volumetric flask, with deionized water as the solvent. Create a seal on the volumetric flask with parafilm, so when the solution is mixed the liquid will not spill out
of the volumetric flask. Placing a finger on top of the parafilm, invert the flask more than three times to mix the solution.Prepare a reference cuvette of deionized water. This reference cuvette helps to create a point at which the MeasureNet can compare the data to. Deionized water is used because it has no ions, which are the light absorbing species. Therefore, the spectroscopy reading for the deionized water should be zero. At the MeasureNet station, prepare for thespectroscopy data by selecting F2, absorption, from the Main Menu. To reset the data values, press display. Transfer the prepared solution to a cuvette via a graduated pipette, filling the cuvette three quarters of the way full, and place a cuvette lid on the cuvette. At the spectrometer, prepare the MeasureNet system by: entering your station number, zeroing it out with the block cuvette, and taking a reference with the deionized water. The block cuvette is to help zero the sensor on the spectroscopy machine out and reset all data about to be used. The reference cuvette is to give a base level for comparison, and to make sure the machine is reading properly because the readings for absorption should be zero. Put all cuvettes into the spectroscopy machine with the clear, smooth sides in the direction the light will come from. Make sure to clean all three cuvettes with a Kimwipe. Then place your sample in the spectrometer and take a reading of the light that was absorbed. Have the person at the MeasureNet station check the results for accurate absorbance readings. If the data is good, which is a normal distribution graph, more simply bell shaped,at the MeasureNet, save the data via file options with F3 and save in a three-digit code with the first two numbers of the MeasureNet station number, 02, and the last one being the number of the sample just run. If the data does not have a significant bell shape to it,
or one at all, re-reference the machine and run the data again. Even though the samples

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture