Understand the Tiseliust and Kabat Experiment Understand Ig Superfamilies

Understand the tiseliust and kabat experiment

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- Understand the Tiseliust and Kabat Experiment - Understand Ig Superfamilies - Understand the functions of CDR’s - Understand Isotypes, Allotypes and Idiotypes - Know the basic idea of variable domain affinity (K off and K on ) - Understand how Fc-receptors work. - Know ADCC - Understand BCR and co-receptors Lecture-4: Experimental Immunology - Understand how to make a recombinant clone and how to get a protein from that clone. You should know how to select for the clone and test for the presence of the gene, mRNA protein and regulatory regions. Basic molecular techniques should be reviewed very carefully. Make sure you understand vectors and how they are used - Know when and why you use a PAGE vs. agarose gel - Know when and how to label nucleic acids and proteins, when you need a label and how will it help you in what you're looking for. - Understand Fluorescent staining and FACS. - Know when and how to use the different blots. Southern, Northern and Western. - Understand when and how to use ELISA’S, Immunoprecipitation, affinity tags, cell lines.
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  • Summer '10
  • innate response, Immunology Study Guide, main signaling proteins, Basic molecular techniques, variable domain affinity

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