analyte (already separated from the other analytes in the chromatographic mixture)denoted as ABC exiting the chromatographic column and: the analyte (A-B-C) undergoing ionization and fragmentation the charged fragments (A+B+C+) being separated by mass the fragments which are focused on the mass filter's exit slit passing into thedetector and the charged ions being detected. In this example, the lightest fragment is B+; the heaviest A+. The last frame of themovie is a mass spectrum displaying only these three fragments. Their relative massto charge ratios are specified by their relative position on the x axis (lowmass/charge to left, high mass/charge to right). The relative amounts (commonlycalled peak intensity) of each of these fragments determined during the massanalyzer's scan is reflected by the y axis. The QuickTime move and GIF animations are both available here:These notes were written by Dr. Thomas G. Chasteen at Sam Houston StateUniversity, Huntsville, Texas.
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