for Lineweaver-Burke, a reciprocal is taken from the estimates that are calculated for Vmax and Km, with the resulting error value being greater than compared to a non-linear regression curve. This is also because the parameters that are used in the non-linear regression, and the error for these parameters is taken from the individual data points for a more accurate plot. Since the error is greater in the linear plot, the non-linear regression is preferred. The Kcat values for this experiment showed that this type of inhibitor was most likely a competitive inhibitor. The Vmax value itself was significantly lower than the Vmax value for the enzyme when half was used. When using half the amount of enzyme, the amount of substrate was more than the amount of full enzyme being used at one point as well, and this was different
from the rate when an inhibitor was present in the mix. With the inhibitor present, the amount of product was even lower compared to when it was the full amount of enzyme. These data further support the possibility of this being a competitive inhibitor. It also appears that with half the amount of enzyme present, the enzyme was almost appearing to be more uninhibited because of the higher amount of product being present at the end. Concluding from this experiment, the results suggest that overall, this was a competitive inhibitor used in the continuous kinetic assay. Compared to when a full amount of enzyme was
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