Tie off the other end of the bag Leave sufficient space for the expansion of

Tie off the other end of the bag leave sufficient

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Remove most of the air from each bag by drawing the dialysis bag between two fingers. Tie off the other end of the bag. Leave sufficient space for the expansion of the contents in the bag. (The Solution should fill only about one-third to one-half of the piece of tubing.) 3. Rinse each bag gently with distilled water to remove any sucrose spilled during filling. 4. Carefully blot the outside of each bag and record (Table 1.2) the initial mass of each bag, expressed in grams. 5. Fill six 250-mL beakers or cups two-thirds full with distilled water. 6. Immerse each bag in one of the beakers of distilled H O and label the beaker to indicate the molarity of the solution in the dialysis bag. Be sure to completely submerge each bag.
7. Let them stand for 30 minutes. 8. At the end of 30 minutes remove the bags from the water. Carefully blot and determine the mass of each bag. 9. Record your groups’ data (Table 1.2). Obtain data from the other lab groups in your class to complete (Table 1.3) Class Data. Observations Table 1.1: Solution Color Presence of Glucose Initial Contents Initial Final Initial Final Bag 15% glucose & 1% starch White Black Yes Yes Beaker H O + IKI Amber Amber No No Table 1.2: Individual Data Contents in Dialysis Bag Initial Mass Final Mass Mass Difference Percent Change in Mass a) Distilled Water 25.3g 24.6g 0.7g 2.78% b) 0.2 M 26.0g 26.8g 0.8g 3.09% c) 0.4 M 27.7g 29.3g 2.5g 9.34% d) 0.6 M 27.7g 30.4g 2.7g 9.76% e) 0.8 M 27.4g 32.7g 5.3g 19.35% f) 1.0 M 27.8g 34.0g 6.2g 22.31% Table 1.3 Dialysis Bag Results: Class Data Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 Group 8 Total A Distilled water 0 2.66% 1.9% 0 .81% 0 5.00% 2.76% 13.13% 0.2M 8.7% 6.64% 4.65% 4.53% 4.82% 11.77% 3.75% 3.09% 47.95%
0.4M 10.4% 8.77% 19.5% 10.22% 9.33% 15.25% 8.03% 9.34% 90.84% 1 0.6M 14.2 15.38 6.11 13.55 9.23 22.28 18.82 9.76 109.33% 1 0.8M 16.4 17.21 16.6 29.92 15.55 17.27 21.8 19.35 154.1% 1 1.0M 21.03 15.08 17.8 30.43 18.05 33 18.3 22.31 176% Class Data Graph: Results

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