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Remove most of the air from each bag by drawing the dialysis bag between two fingers. Tie off the other end of the bag. Leave sufficient space for the expansion of the contents in the bag. (The Solution should fill only about one-third to one-half of the piece of tubing.)3.Rinse each bag gently with distilled water to remove any sucrose spilled during filling.4.Carefully blot the outside of each bag and record (Table 1.2) the initial mass of each bag, expressed in grams.5.Fill six 250-mL beakers or cups two-thirds full with distilled water.6.Immerse each bag in one of the beakers of distilled H₂O and label the beaker to indicate the molarity of the solution in the dialysis bag. Be sure to completely submerge each bag.
7.Let them stand for 30 minutes.8.At the end of 30 minutes remove the bags from the water. Carefully blot and determine the mass of each bag.9.Record your groups’ data (Table 1.2). Obtain data from the other lab groups in your class to complete (Table 1.3) Class Data.ObservationsTable 1.1:Solution ColorPresence of GlucoseInitial ContentsInitialFinalInitialFinalBag15% glucose & 1% starchWhiteBlackYesYesBeakerH₂O + IKIAmberAmberNoNoTable 1.2: Individual DataContents in Dialysis BagInitialMassFinalMassMass DifferencePercent Change in Massa)Distilled Water25.3g24.6g0.7g2.78%b)0.2 M26.0g26.8g0.8g3.09%c)0.4 M27.7g29.3g2.5g9.34%d)0.6 M27.7g30.4g2.7g9.76%e)0.8 M27.4g32.7g5.3g19.35%f)1.0 M27.8g34.0g6.2g22.31%Table 1.3 Dialysis Bag Results: Class DataGroup 1Group 2Group 3Group 4Group 5Group 6Group 7Group 8TotalADistilled water02.66%1.9%0.81%05.00%2.76%13.13%0.2M8.7%6.64%4.65%4.53%4.82%11.77%3.75%3.09%47.95%
0.4M10.4%8.77%19.5%10.22%9.33%15.25%8.03%9.34%90.84%10.6M14.215.386.1113.559.2322.2818.829.76109.33%10.8M16.417.2116.629.9215.5517.2721.819.35154.1%11.0M21.0315.0817.830.4318.053318.322.31176%Class Data Graph:Results