Directly linked to students daily lives 27forensic

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Directly linked to students daily lives
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27 Forensic DNA Fingerprinting Compare Crime Scene DNA with 5 Suspects to determine “Who Done it?” Restriction Analysis of Lambda DNA Use restriction enzymes and gel electrophoresis to analyze DNA LIFE SCIENCE STANDARDS & SKILLS: Allelic differences in populations DNA as a unique identifier – Heredity Restriction enzymes Function and properties Origin in bacteria and use in genetic engineering Plasmid mapping Agarose Gel Electrophoresis Separation of molecules using electrical current Size based on distance traveled, creating buffers Micropipetting techniques
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28 Crime Scene Investigator PCR Basics™ Students learn to use the polymerase chain reaction (PCR) and DNA electrophoresis to determine the genotypes of 5 DNA samples Simulate real-world crime lab techniques using one of the Short Tandem Repeat (STR) loci commonly used in forensic typing. LIFE SCIENCE STANDARDS & SKILLS: Allelic differences in populations DNA as a unique identifier Use of PCR in DNA profiling Polymorphic loci and multiple alleles Non-coding DNA sequences Agarose Gel Electrophoresis Separation of molecules using electrical current Size based on distance traveled, creating buffers Micropipetting techniques
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29 PV92 PCR Informatics Students isolate and examine the DNA fingerprint of their own DNA LIFE SCIENCE STANDARDS & SKILLS: Population Genetics Hardy Weinberg equilibrium Evolution, migration, speciation Bioinformatics using Cold Spring Harbor allele server Polymerase Chain Reaction Uses in forensics, archeology, research, disease diagnostics Agarose Gel Electrophoresis Separation of molecules using electrical current Size based on distance traveled, creating buffers
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30 GMO Investigator™ Students extract and amplify DNA from grocery store foods LIFE SCIENCE STANDARDS & SKILLS: Agriculture and environment – Pesticides and herbicides – Population growth and environmental challenges – Plant biodiversity and ecosystems Molecular Biology – DNA replication and PCR Genetic transformation to create GMOs – Control of gene expression (in foreign hosts)
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31 Got Protein?™ Kit Determine the protein concentration in common beverages such as milk, sports drinks and more Engages students – they bring in their samples of choice LIFE SCIENCE STANDARDS & SKILLS: Proteins Essential molecules in all living cells Bradford Assay Coomassie Blue dye binds to protein and allows for quantitation and detection correlation between the amount of blue color and the amount of protein Spectrometry Use of spectrophotometer Apply Beer’s Law Inquiry based - which foods/drinks provide more proteins? Directly links to students daily lives
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32 ELISA Immuno Explorer™ Students simulate spreading an infectious disease and detect origin LIFE SCIENCE STANDARDS & SKILLS: Immunology antigen-antibody interaction – virology infectious diseases Enzyme Properties enzyme-substrate interaction colorimetric detection Real world applications Pregnancy, drug, HIV testing
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