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Lab__3a_Preparing_Bacterial_Smear_for_Differential_Staining

Also include an a to identify that slide will be acid

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Also include an “A” to identify that slide will be Acid-fast stained. Flip slide over. Use DI water dropper to place very small drop of water inside each circle. Using a sterilized inoculation loop , take a small sample of your unknown. Be gentle! The bacteria is on the surface of the medium. Swirl into the water in the center circle of your slide. Q: What (+) and (-) control can we use for this stain? Use same method to add controls to circle on left and right. Heat fix the slide on top of your microincinerator . Allow it to stay in the platform for 5 minutes after water has completely evaporated. From the Virtual Microbiology Classroom on ScienceProfOnline.com Watch video of How to Prepare a Bacterial Smear for Acid Fast Staining
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Endospore Stain Distinguishes between two things: - endospores, which stain green - vegetative cells, which stain pink Q: What is an endospore? Q: What two genera of endospore-producing bacteria have we studied in class? + u - E To prepare Endospore bacterial smear for staining next week: Draw three circles on slide using wax pen. Also include an “E”to identify that slide will be Endospore stained. Flip slide over. Use DI water dropper to place very small drop of water inside each circle. Using a sterilized inoculation loop , take a small sample of your unknown. Be gentle! The bacteria is on the surface of the medium. Swirl into the water in the center circle of your slide. Q: What (+) and (-) control can we use for this stain? Use same method to add controls to circle on left and right. Heat fix the slide on top of your microincinerator . Allow it to stay in the platform for 5 minutes after water has completely evaporated.
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