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One particularly unwelcome bacillus species forms

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these Colonies for subsequent Analysis. One particularly unwelcome Bacillus Species forms spreading, swirling Colonies that will rapidly overgrow every other Bacterium on your Plate. This is the dreaded Bacillus mycoides (G= bacillus like a fungus) . Get rid of it! _________ “I love you.” I know.’ © 1980 LucasFilm Ltd.
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Lab 2 Page 22 Skin Bacteria Your Micrococcus luteus or Staphylococcus epidermidid was a Direct Isolation from a “Patient’s” Skin Swab onto an Indicator Medium. The “Indicators” in YGC Plates are the Production of Pigment and the Production of Acid resulting in a Zone of Clearing surrounding Colonies. Micrococcus luteus . (L= small berries yellow) produces Yellow-Pigmented Colonies that don’t produce large Amounts of Acid on Glucose and so are not surrounded by a Zone of Clearing. Micrococcus luteus is a harmless Commensal Bacterium (L= dines with) in your Skin Flora. That being said, it’s best to treat any Bacterial Culture (Soil, Skin or Enteric) as though it were Pathogenic... Staphylococcus epidermidis (L= clustered berries of the skin) produces White- Pigmented Colonies that produce large Amounts of Acid on Glucose and so are surrounded by a Zone of Clearing. Staphylococcus epidermidis is another harmless Commensal Bacterium in your Skin Flora. Staphylococcus aureus (L= clustered golden berries) produces Yellow-Pigmented Colonies surrounded by a Zone of Clearing. Staphylococcus aureus is a Pathogenic Bacterium in your Skin Flora. Any Plates containing Yellow Colonies surrounded by a Zone of Clearing should be placed into Oscar. After confirming Colony Morphology use your Inoculating Loop to gather Bacteria from the Edge of an Isolated Colony. Remember, just about anything can and will grow on YGC Plates, so don’t be shocked if you have Fungi besides Yeast on your Plates. Penicillium is a common Isolate that can be preliminarily identified by its characteristic dark Sage Color. Enteric Bacteria (Atlas Figures 2-15 and 2-16, Page 12) The EMB Plate is for Identification of your Enteric. Don’t use it for Gram Staining or Streaking. Your Nutrient Agar Plate is for Gram Staining and Streaking. Your EMB Plate should have either Purple Colonies surrounded by a Fly-Butt Green Metallic Sheen (E. coli ) or Hot Pink Colonies (Enterobacter ). You might notice that some of the more isolated Colonies aren’t surrounded by any Fly-Butt Green Metallic Sheen or aren’t Hot Pink. This is because they’re isolated Colonies and have not achieved the Critical Mass necessary to produce sufficient Acid to precipitate the Dyes in EMB. Use an isolated Enteric Colony on your Nutrient Agar Plate to streak a new Nutrient Agar Plate. ____________________________________________________________ The force is with you, young Skywalker, but you are not a Jedi yet. © 1980 LucasFilm Ltd.
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