In vitro technique used to repeatedly duplicate a

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In vitro technique used to repeatedly duplicate a specify region of a DNA molecule increasing the number of copies exponentially Copies are the pcr product Rt-pcr A modification of pcr used to detect certain mRNA sequences ; the method relies on reverse transcriptase to generate cDNA from the mRNA, and that cDNA is then amplified exponentially Qpcr A modification of pcr that modifies the pcr products accumulation allowing the researcher track the amplification in real time and determine the relative amount of target initially in the sample; also called real time pcr Key ingredients for pcr methods Double stranded DNA
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Taq polymerase Primers Deoxynucleotides DNA probes Single stranded piece of DNA tagged with an identifiable marker that is used to detect a complementary sequence Hybridization The annealing of two complementary strands of DNA from different sources to create hybrid double stranded molecule Colony blotting Technique used to determine which colonies on an agar plate contain a given nucleotide sequence Fluorescence in situ hybridization Technique used to detect a given nucleotide sequence within intact cells on a microscope slide DNA microarray A probe based technique used to study gene expression patterns
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  • Fall '19
  • DNA, recombinant DNA molecules

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