Tina stands for twisted intercalating nucleic acid it

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TINA stands for Twisted Intercalating Nucleic Acid. It is a novel molecule for labelling PCR primers at the 5’ end. A stable performance over a wide range of primer concentrations and annealing temperatures is ensured with TINA. PCR assays achieve increased sensitivity without compromising their specificity! TINA primers are HPLC purified for supreme quality and available in 5 synthesis scales for any project size. Achieving Better Results Using TINA primers improves the overall performance of PCR assays. Improved efficiency & kinetics Particularly whenever primers are the limiting factor Enhanced overall specificity & sensitivity Increased probaility of enhanced analytical and clinical sensitivity Increased robustness PCRs tolerate impure sample material, withstand exo- nucleases, and endure high background genomic DNA (see Fig. 2) Improved PCR Assays. Fig.2 Amplification of an octaplex end-point PCR by unmodified primers and 5’-TINA modified primers. The orange box highlights the lack of amplicons for the gene with unmodified primers. Read the TINA application note for more details. Fig.1 Comparison of Cp / Ct values after Real-Time PCR with non – modified oligos (0.5 μM) and TINA modified oligos (0.25 to 0.5 μM). Read the TINA application note for more details.
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