Gatorade Lab Packet (1) (1) (1) (3).docx

3 once you have collected all seven click stop then

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3. Once you have collected all seven, click Stop. Then click the Linear Fit icon on the toolbar to add a trend line. Note the slope of the line (Hint- remember “m” denotes slope) as it appears in the text box.
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Testing a bottled sample: Now that you have created a reference chart with various standards, you will test a sample of bottled Gatorade to determine how much powder mix is necessary to make the bottled Gatorade. 1. Obtain a bottled sample from the instructor and add 3 ml in a cuvette. Put the cuvette in the spectrometer. 2. Click Collect (and Store Latest Run) and run the assay on the bottled Gatorade. You should see a new dot on your reference chart. Click keep to store the point. Now hit stop. 3. Now using the absorbency of the bottled Gatorade figure out the percent solution of the sample. In other words, how much powder did the Gatorade factory use when making the bottled Gatorade? Determine the percent solution either visually using the graph, or by using the equation of the best fit line that was created. ***Hint- Do not go directly vertical to the x-axis for the value. If you need direction, ask your instructor or peers for pointers.*** 4. Try to re-create the bottled Gatorade percent solution by measuring out the appropriate amount of Gatorade powder using the scales in the back of the room to match the percent solution of the bottled sample. 5. Put 3 ml of your recreated Gatorade into a cuvette and put the cuvette into the spectrometer. Hit Collect (and Store Latest Run). You should now see another dot on your reference chart. Hit Stop. If the dot is in the same location as the bottled Gatorade’s dot, then you have successfully recreated the same percent solution Gatorade as bottled Gatorade. 6. Record the amount of Gatorade mix necessary to re-create the bottled sample. 7. Make sure you screen shot your absorbency vs. percent solution graph before you exit logger pro.
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  • Spring '17
  • Baker, Lanning R,
  • Gatorade, Laboratory glassware, Percentage solution

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