Coordinated water molecule pointing outward is

Info icon This preview shows pages 96–101. Sign up to view the full content.

View Full Document Right Arrow Icon
coordinated water molecule (pointing outward) is hydrogen-bonded to Glu- 270. On the opposite side of Glu-270 the three arginines (Arg-145, Arg-127, and Arg-7l) can be seen, more or less on a vertical line, running from top to bottom of the figure. In the upper part of the figure, pointing outward, is the aromatic ring of Tyr-248. Behind it is the hydropho- bic pocket. C-5
Image of page 96

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
C-6 Figure 2.34 Liver alcohol dehydrogenase subunit viewed as a CPK model. The left-hand side of the mole- cule is the coenzyme binding domain and the right-hand side is the catalytic domain. The cata- lytic zinc ion is accessible from two channels located above (not visible) and below the coen- zyme binding domain. The upper channel permits approach of the nicotinamide ring of the coenzyme. The lower channel permits approach of the substrate. The substrate channel closes up, trapping the substrate inside the molecule, when both coenzyme and substrate are present.
Image of page 97
C-7 Figure 3.4 A Fura-2 study of the transient Ca 2 + fluxes in an egg of the sea urchin (Lutechinus pictus). The diameter of this egg is about 120 p.M. The fluorescent dye was injected into the egg, and the fluorescence intensity with excitation at 350 and 385 nm was measured with a lower-light-Ievel television camera feeding a digital image processor (512 x 486 pixels). The image finally dis- played in pseudocolor is the ratio of intensities at the two excitation wavelengths. The series of images shows a wave of high Ca 2 + concentration that traverses the egg after it is fertilized by a sperm. Resting Ca2+ concentration is typically 100 nM and uniform through the cell. The fertil- izing sperm sets off a transient wave of high Ca 2 + that begins as a local elevation and thereafter spreads rapidly. After 20-30 seconds, the Ca 2 + concentration of the entire egg is uniformly high (-2 p.M). The figure is from an experiment by M. Poenie, J. Alderton, R. Steinhardt, and R. Tsien; see also Reference 26.
Image of page 98

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
C-8 CA AND SI COt1PAR I SON t'1ANGAt~ESE I f ~ -, ", I " - Figure 3.7 The elemental distribution of Ca, Si, and Mn in the hair of the common stinging nettle (Urtica dioica) obtained using the Oxford University PIXE microprobe. The color code for Ca is: yel- low, >3.4 M; orange, 2.0-3.4 M; red, 1.5-2.0 M; dark blue, 1-1.5 M; blue, 0.5-1.0 M; light blue, 0.1-0.5 M; white, <0.1 M. The color codes of Si and Mn are similar. The PIXE data show that the tip mainly is made up of Si (presumably amorphous silica), but the region behind is largely made up of Ca (calcium oxalate crystals). The base of the hair contains substantial amounts of Mn. The pictures were kindly provided by R. J. P. Williams.
Image of page 99
C-g Figure 3.17 Space-filling stereo model of bovine brain calmodulin. Residues 5 to 147 are included, N-termi- nal half at the top. Positively charged side chains (Arg, Lys, His) are dark blue, negatively charged (Asp, Glu) red, hydrophobic (Ala, Val, Leu, lie, Phe) green, Met yellow, Asn and Gin purple, Ser, Thr, and Tyr orange, and Pro, Gly, and main-chain atoms light blue. The figure was kindly provided by Y. S. Babu et al.
Image of page 100

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 101
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern