The acronym hplc stands for 32 a hydrophobic protein

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31.The acronym HPLC stands for32.A) hydrophobic protein liquid chromatography.33.B) high performance liquid chromatography.34.C) hydrophilic partition liquid chromatography.35.D) high priced liquid chromatography.36.E) hydrostatic process liquid chromatography.Ans: B
Level of Difficulty: EasySection: 5.2.CLearning objective: Protein Purification and Analysis32.A technique that can be used to separate proteins based primarily on the presence of non-polar residues on their surface is called
Level of Difficulty: EasySection: 5.2.CLearning objective: Protein Purification and Analysis33.A technique that can be used to separate proteins based primarily on their pIis called34.
Level of Difficulty: EasySection: 5.2.CLearning objective: Protein Purification and Analysis34.Which of the following amino acids would be lastto elute at pH 8.0 from an anion-exchange column?
Level of Difficulty: ModerateSection: 5.2.CLearning objective: Protein Purification and Analysis
35.Which of the following amino acids would be firstto elute at pH 8.0 from an anion-exchange column?36.A) lysine37.B) alanine38.C) glutamic acid39.D) asparagine40.E) glycineAns: A
Level of Difficulty: ModerateSection: 5.2.CLearning objective: Protein Purification and Analysis36.The pK1, pK2, and pKRof the amino acid lysine are 2.2, 9.1, and 10.5, respectively. The pK1,pK2, and pKRof the amino acid arginine are 1.8, 9.0, and 12.5, respectively. A student at SDSU wants to use ion exchange chromatography to separate lysine from arginine. What pH is likely to work best for this separation?
Level of Difficulty: DifficultSection: 5.2.CLearning objective: Protein Purification and Analysis37.The pK1, pK2, and pKRof the amino acid histdine are 1.8, 9.3, and 6.0, respectively. The pK1, pK2, and pKRof the amino acid arginine are 1.8, 9.0, and 12.5, respectively. You have a mixture of histidine and arginine, how would you try to separate these two amino acids?38.
Level of Difficulty: DifficultSection: 5.2.CLearning objective: Protein Purification and Analysis

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