Transcription is a chemical reaction: General Features of RNA Synthesis: Similar to DNA synthesis except the precursors are rNTPs and only one strand of DNA is used as a template. Also, RNA chains can be initiated de novo (no primer required). The RNA molecule will be complementary to the DNA template (anti-sense) strand and identical (except that uridine replaces thymidine) to the DNA non-template (sense) strand.
Initiation by RNA Polymerase II: Promoter sequence elements bind transcription factors in an orderd sequence. Basal transcription factors (TFII D, A, B, F) form a pre-initiation complex. Prepares DNA for unwinding and recruits RNA polymerase and (TFIIE) to form a transcription bubble and initiate transcription. RNA polymerase II can move through nucleosomes (FACT protein: modifies histones to allow polymerase II to transcribe).
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- Fall '13
- DNA, DNA Damage